Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 40 µM
Product nameL-Amino Acid Assay Kit
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Sensitivity<= 40 µM
Assay time0h 30m
L-Amino Acid Assay Kit ab65347 is used measure the level of L-amino acids in biological samples. There is no requirement for sample pretreatment or purification.
The L-amino acid(s) level can be quantified using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at λ = 570 nm) methods in 96-well plates.
Amino acid assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with microplate reader
L-Amino acids are the most essential elements in biology. Accurately quantitating L-amino acids in body fluids or purified samples may provide valuable information for diagnostic or basic research studies.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests L-Amino Acid Assay Buffer WM 1 x 25ml L-Amino Acid Enzyme Mix Green 1 vial L-amino Acid Probe Red 1 x 0.2ml L-Amino Acid Standard (4 nmol/µl) Yellow 1 x 300µl
RelevanceAmino acids perform various important functions for the body and serve as the materials for the body's cells, hormones, and enzymes. Of the standard amino acids, all by glycine can exist in either of the two optical isomers, called D and L enantiomers, which are mirror images of each other. The L-amino acids represent the vast majority of amino acids found in proteins during translation in the ribosome, and are therefore the most essential element in biology and metabolic functions.
ab65347 has been referenced in 7 publications.
- Modvig IM et al. Peptone-mediated glucagon-like peptide-1 secretion depends on intestinal absorption and activation of basolaterally located Calcium-Sensing Receptors. Physiol Rep 7:e14056 (2019). PubMed: 31020803
- Galsgaard KD et al. Glucose and amino acid metabolism in mice depend mutually on glucagon and insulin receptor signaling. Am J Physiol Endocrinol Metab 316:E660-E673 (2019). PubMed: 30807215
- Wewer Albrechtsen NJ et al. Hyperglucagonemia correlates with plasma levels of non-branched-chain amino acids in patients with liver disease independent of type 2 diabetes. Am J Physiol Gastrointest Liver Physiol 314:G91-G96 (2018). PubMed: 28971838
- Galsgaard KD et al. Disruption of glucagon receptor signaling causes hyperaminoacidemia exposing a possible liver-alpha-cell axis. Am J Physiol Endocrinol Metab 314:E93-E103 (2018). PubMed: 28978545
- Posada IMD et al. Opposite feedback from mTORC1 to H-ras and K-ras4B downstream of SREBP1. Sci Rep 7:8944 (2017). PubMed: 28827765
- Zhou J et al. Inhibition of LIN28B impairs leukemia cell growth and metabolism in acute myeloid leukemia. J Hematol Oncol 10:138 (2017). PubMed: 28693523
- Zhang Y et al. Coordinated regulation of protein synthesis and degradation by mTORC1. Nature N/A:N/A (2014). PubMed: 25043031