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Do you have any recommendations on the volume of serum I should use in this assay? I have mouse serum samples.
Asked on Apr 13 2012
I general, werecommend using several doses of your serum sample to ensure the readings are within the standard curve range. In essence, the dose range selection depends on your expectation of the L-amino-acid concentration in your samples.
An example of a range of serum volumes you may try is : 0, 1, 5, 10, 25 and 50ul/well.
I hope this is helpful. Please contact us again if you have any further questions.
Answered on Apr 13 2012