Question (43719) | L-Amino Acid Assay Kit (ab65347)

I general, werecommend using several doses of your serum sample to ensure the readings are within the standard curve range. In essence, the dose range selection depends on your expectation of the L-amino-acid concentration in your samples.

An example of a range of serum volumes you may try is : 0, 1, 5, 10, 25 and 50ul/well.

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