Key features and details
- Detection method: Colorimetric
Product nameL-Arginine Assay Kit
The L-Arginine Assay Kit (ab241028) provides a quick, specific, and easy method for the measurement of total L-arginine concentrations in a wide variety of samples. In this enzyme-based assay, L-arginine is converted into a series of intermediates, which will further react with a probe producing a stable colorimetric signal (OD: 450 nm).
The kit is simple to use, sensitive and high-throughput adaptable and can detect as low as 1 nmol/well of L-arginine in biological samples.
This product is manufactured by BioVision, an Abcam company and was previously called K749 L-Arginine Assay Kit (Colorimetric). K749-100 is the same size as the 100 test size of ab241028.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Arginine Assay Buffer 1 x 25ml Arginine Enzyme Mix 1 vial Arginine Probe Mix A 1 x 12ml Arginine Probe Mix B 1 x 12ml Arginine Standard 1 vial Sample Cleanup Mix 1 vial
L-arginine standard curve.
Specificity of the detection of L-arginine over other amino acids: D-arginine and other L-amino acids were tested at a 2-fold molar excess (each AA: 40 nmol) vs L-arginine (20 nmol).
Estimations of L-arginine in two human serum sample (30 µL) and orange juice (1.6 µL). L-arginine concentrations were 0.278 mM and 0.069 mM in the two human serum samples, and 5.113 mM in orange juice. Assays were performed following the kit protocol.
ab241028 has been referenced in 2 publications.
- Zhao Z et al. Pegylated Recombinant Human Arginase 1 Induces Autophagy and Apoptosis via the ROS-Activated AKT/mTOR Pathway in Bladder Cancer Cells. Oxid Med Cell Longev 2021:5510663 (2021). PubMed: 33791071
- Hou Q et al. Exogenous L-arginine increases intestinal stem cell function through CD90+ stromal cells producing mTORC1-induced Wnt2b. Commun Biol 3:611 (2020). PubMed: 33097830