Overview

  • Product name

    L-Arginine Assay Kit
  • Detection method

    Colorimetric
  • Product overview

    The L-Arginine Assay Kit (ab241028) provides a quick, specific, and easy method for the measurement of total L-arginine concentrations in a wide variety of samples. In this enzyme-based assay, L-arginine is converted into a series of intermediates, which will further react with a probe producing a stable colorimetric signal (OD: 450 nm).


    The kit is simple to use, sensitive and high-throughput adaptable and can detect as low as 1 nmol/well of L-arginine in biological samples.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Arginine Assay Buffer 1 x 25ml
    Arginine Enzyme Mix 1 vial
    Arginine Probe Mix A 1 x 12ml
    Arginine Probe Mix B 1 x 12ml
    Arginine Standard 1 vial
    Sample Cleanup Mix 1 vial

Images

  • L-arginine standard curve.
  • Specificity of the detection of L-arginine over other amino acids: D-arginine and other L-amino acids were tested at a 2-fold molar excess (each AA: 40 nmol) vs L-arginine (20 nmol).
  • Estimations of L-arginine in two human serum sample (30 µL) and orange juice (1.6 µL). L-arginine concentrations were 0.278 mM and 0.069 mM in the two human serum samples, and 5.113 mM in orange juice. Assays were performed following the kit protocol.

Protocols

References

ab241028 has not yet been referenced specifically in any publications.

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