Recombinant
RabMAb

Anti-L-Arginine decarboxylase antibody [EPR7124(2)] (ab157214)

Overview

  • Product name
    Anti-L-Arginine decarboxylase antibody [EPR7124(2)]
    See all L-Arginine decarboxylase primary antibodies
  • Description
    Rabbit monoclonal [EPR7124(2)] to L-Arginine decarboxylase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to amino acid residues in Human L-Arginine decarboxylase was used as an immunogen (UniProt ID Q96A70).

  • Positive control
    • Human cerebellum lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab157214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Decarboxylates L-arginine to agmatine. Truncated splice isoforms probably lack activity.
    • Tissue specificity
      Highly expressed in brain. Also expressed in testis.
    • Sequence similarities
      Belongs to the Orn/Lys/Arg decarboxylase class-II family.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADC antibody
      • ADC_HUMAN antibody
      • Antizyme inhibitor 2 antibody
      • ArgDC antibody
      • Arginine decarboxylase antibody
      • AZI2 antibody
      • KIAA1945 antibody
      • L-Arginine carboxy lyase antibody
      • ODC antizyme inhibitor 2 antibody
      • ODC paralog antibody
      • ODC-p antibody
      • ODC-paralogue antibody
      • ODC1L antibody
      • ODCp antibody
      • Ornithine decarboxylase like antibody
      • Ornithine decarboxylase paralog antibody
      • Ornithine decarboxylase-like protein antibody
      see all

    Images

    • Overlay histogram showing SH-SY5Y cells stained with ab157214 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab157214, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Anti-L-Arginine decarboxylase antibody [EPR7124(2)] (ab157214) at 1/1000 dilution + Human cerebellum lysate at 10 µg

      Predicted band size: 50 kDa

    References

    ab157214 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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