Anti-L-Aspartate antibody (ab9439)

Submit a review Q&A (2)References (1)

Key features and details

  • Rabbit polyclonal to L-Aspartate
  • Suitable for: IHC-FoFr, IHC-Fr, ICC, Immunomicroscopy
  • Reacts with: Species independent
  • Isotype: IgG

Overview

  • Product name

    Anti-L-Aspartate antibody

  • Description

    Rabbit polyclonal to L-Aspartate

  • Host species

    Rabbit

  • Specificity

    The antibody is calibrated against a spectrum of antigens to assure hapten selectivity. No measurable cross-reactivity (<1:1000)was detected against aspartate in peptides or proteins. Fixed tissue cross-reactivity was tested with known targets at the recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine, gamma-aminobutyrate, agmatine, guanidine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Significant cross-reactivity (1:10) was detected against D-aspartate.

  • Tested applications

    Suitable for: IHC-FoFr, IHC-Fr, ICC, Immunomicroscopymore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Chemical/ Small Molecule by a Glutaraldehyde linker.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.
Notes
IHC-FoFr
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Immunomicroscopy
Use at an assay dependent concentration.

Target

  • Relevance

    Aspartic acid (Asp), also known as aspartate, is one of the 20 natural proteinogenic amino acids which are the building blocks of proteins. It is non-essential in mammals, and might serve as an excitatory neurotransmitter in the brain. It is also a metabolite in the urea cycle and participates in gluconeogenesis. As a neurotransmitter, aspartic acid may provide resistance to fatigue and thus lead to endurance, although the evidence to support this idea is not strong.

  • Alternative names

    • Aspartate antibody
    • Aspartic acid antibody
    • L ASP antibody
    • L aspartate antibody

References (1)

Publishing research using ab9439? Please let us know so that we can cite the reference in this datasheet.

ab9439 has been referenced in 1 publication.

  • Sun D  et al. Metabolic and functional profiling of the normal rat retina. J Comp Neurol 505:92-113 (2007). IHC ; Rat . PubMed: 17729258

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Hello, I have a question about Anti-L-Aspartate antibody (ab9439). Would this Anti-L-Aspartate antibody work on mouse and/or human tissue?

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Answer


The immunogen of ab9439 is a Chemical / Small Molecule by a Glutaraldehyde linker. This is not species specific and therefore should work on mouse and/or human tissues however, we have not tested this.

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Question

Customer question: Looking to do IHC on paraffin embedded, glutaraldehyde fixed rat tissue for aspartate. Also ab6450 same question: Are these antibodies appropriate for this application

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Answer

I apologize for the delay with answering this question, I have only just heard back for the antibody's originator. Ab9439 - Has been tested on both paraffin embedded and frozen sections; No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine, gamma-aminobutyrate, agmatine, guanidine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamate, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. For more information, please see online datasheet. Ab6450 - Has been tested on on paraffin embedded sections.

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