• Product name
    L-Carnitine Assay Kit
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
  • Assay type
  • Range
    1 µM - 200 µM
  • Assay time
    0h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammals
  • Product overview

    Abcam's L-Carnitine Assay Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as serum. The assay transfers an acetyl group from CoA to carnitine and the free CoA formed is further processed with subsequent oxidation of the OxiRed probe to give fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). The normal range for serum L-Carnitine is ~20-100 µM. The detection sensitivity is ~1 µM for the fluorometric assay and ~10 µM for the colorimetric assay.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Carnitine is a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine. It is required for transport of fatty acids into the mitochondrial matrix via the carnitine/acylcarnitine shuttle where beta-oxidation occurs, acetate is generated and the acetate utilized in the TCA cycle for the generation of energy. L-Carnitine is often sold as a nutritional supplement. Carnitine exists in two stereoisomers. Only L-carnitine is biologically active.


Associated products


  • Fluorimetric standard curve: mean of duplicates (+/- SD) with background readings subtracted.

  • L-Carnitine measured in biologicals showing concentration (micromolar).

  • Example of L-Carnitine standard curve obtained using ab83392.



ab83392 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

L-carnitine concentration was measured using fluorimetry in a yeast strain capable of carnitine biosynthesis, both with and without supplementation with the carnitine precursor gamma-butyrobetaine (gBB).
The yeast were lysed using glass beads in assay buffer. The background was higher than in the fluorimetric standard curve, likely due to interfering metabolites, possibly choline. The increased carnitine detection in gBB-supplemented cells suggests that kit ab83392 can be used for comparative carnitine measurements in cells other than human origin, including in yeasts however, the high background levels complicate absolute quantification.

Abcam user community

Verified customer

Submitted Jun 03 2016

The ab138889 CoA detection kit assay buffer is 100 mM Na phosphate buffer, pH = 6. The RIPA buffer may be an issue with the other two kits, since they are enzyme-based, and that kind of assay is generally incompatible with detergents. Your best approac...

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The instructions in the protocol are correct, and the values are too, 0, 2, 4, 6, 8, and 10 nmol per well. Here is the calculation:

Starting with the 1 mM standard carnitine solution, calculate the moles per microliter.

1 millimol...

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Thank you for contacting us.

We are not ISO registered company which is why we do not have CE certification. We can however provide certificate of analysis, if requested. I don't think CE certification is required for research products. Our pr...

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Thank you for your message and for your patience.

I have already received information to confirm that this kit has been optimized to measure free L-carnitine and so regrettably we cannot guarantee or advice regarding measurement of total L c...

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This kit will be very suitable for use with EDTA plasma samples.

Thank you for your enquiry regarding ab83392.

My colleague Kate has been out of the office this morning. Here are some links which show these levels:



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Thank you for your inquiry.

This kit should be very specific for L-Carnitine since the enzymes used are specific to it. Also the reaction mimics the biological activity of L-Carnitine and only L-Carnitine is active not D-Carnitine.


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Thank you for contacting Abcam

Upon arrival the kit has to be stored at -20C. Certain key components in the kit are lyophilized so the kit may be stable. However I cannot guarantee this and you will have to test whether it is still working. <...

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Thank you for contacting Abcam regarding ab83392. Unfortunately, the specific details of the reaction mechanism and enzyme names are proprietary information in this case. I can only provide the following: Acetyl CoA + Carnitine ---> Free CoA + Acetylat...

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