Overview

  • Product name
    Anti-L Dopa antibody
  • Description
    Rabbit polyclonal to L Dopa
  • Host species
    Rabbit
  • Specificity
    Using a conjugate L-DOPA-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
    CompoundCross-reactivity ratio (a)
    L-DOPA-G-BSA1
    Dopamine-G-BSA1/>50,000
    Noradrenalin-G-BSA1/>50,000
    Tyrosine-G-BSA1/>50,000
    (a): L-DOPA-G-BSA concentration/unconjugated or conjugated catecholamine concentration at half displacement (b): non-reduced conjugate G = glutaraldehyde, BSA = bovine serum albumin
  • Tested applications
    Suitable for: ELISA, IHC-Frmore details
  • Immunogen

    L-dihydroxyphenylalanine (L-DOPA)-Glutaraldehyde-Carriers

  • General notes


    The antiserum was tested using the free floating PAP technique on rat dopaminergic areas. The anti-conjugated L-DOPA antibodies gave a good staining between 1/2000-1/5000 dilution in these areas.

Properties

Applications

Our Abpromise guarantee covers the use of ab6426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/5000.
IHC-Fr 1/2000 - 1/5000.

Target

  • Relevance
    L Dopa is an intermediate in dopamine biosynthesis. Clinically, L Dopa is used in the management of Parkinson's disease. It is used as a prodrug to increase dopamine levels since it is able to cross the blood-brain barrier whereas dopamine itself cannot. Once L Dopa has entered the central nervous system (CNS), it is metabolised to dopamine by aromatic L amino acid decarboxylase. This also occurs in the peripheral tissues, causing adverse effects and decreasing the available dopamine to the CNS, so it is standard practice to co administer a peripheral DOPA decarboxylase inhibitor and often a catechol-O-methyl transferase (COMT) inhibitor.
  • Alternative names
    • 3 4 dihydroxy L phenylalanine antibody
    • L dihydroxyphenylalanine antibody
    • Levodopa antibody

References

This product has been referenced in:
  • Jager M  et al. Evidence for involvement of Wnt signalling in body polarities, cell proliferation, and the neuro-sensory system in an adult ctenophore. PLoS One 8:e84363 (2013). IF . Read more (PubMed: 24391946) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer


You are correct that to produce an antibody to a small molecule it needs to be conjugated to a larger protein to enhance the immune response. ab6426 will detect will detect DOPA in tissues. In the Primary antibody note on the online datasheet, it states "The antiserum was tested using the free floating PAP technique on rat dopaminergic areas. The anti-conjugated L-DOPA antibodies gave a good staining between 1/2000-1/5000 dilution in these areas."

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Answer

Thank you for your email and I understand your concern. Ab5979 was originated and characterized outside Abcam. The reason why L-dopa was conjugated to glutaraldehyde and BSA was to make the protein more immunogenic. However, this exact process is the intellectual property of the antibody's originator (basically, this is to prevent someone else from making the antibody themselves. I don't have access to this information and if I did I would forward it to you). The antibody was tested in an ELISA against the immunogen to ensure specificity. The antibody will detect L-dopa, not just the conjugate L-DOPA-Glutaraldehyde-Protein. The antibody will not recognize BSA or Glutaraldehyde alone. Please contact us again if you have any additional questions.

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Question
Answer

Yes, this antibody can also recognize human DOPA.

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Question
Answer

Yes, anti-L-Dopa antibody can recognize L-Dopa in avian tissue.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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