Overview

  • Product name
  • Description
    Chicken polyclonal to L Glutamate
  • Host species
    Chicken
  • Specificity
    No measurable cross-reactivity (<1:1000) against glutamate in peptides or proteins. Fixed tissue cross-reactivity tested with known targets at recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against L-alanine,gamma-aminobutyrate, D/L-aspartate,1-amino-4-guanidobutane (AGB), D/L-arginine, L-citrulline, L-cysteine, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Modest cross-reactivity (1:20) against D-glutamate. Significant cross-reactivity (1:8) against free NAAG in competition assays (NAAG is not retained in aldehyde-fixed tissue).
  • Tested applications
    Suitable for: IHC - Wholemount, ICCmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    L Glutamate cross-linked to purifed fraction V bovine serum albumin with glutaraldehyde.

Properties

Applications

Our Abpromise guarantee covers the use of ab62668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC - Wholemount Use at an assay dependent concentration.
ICC 1/100. Endogenous content mapping by LM and EM immunocytochemistry. True dilution at user dilution: 1/32000. Optimal fixation: 0.1-2.5% glutaraldehyde,1% formaldehyde using HPI (High Performance Immunocytochemistry). Minimum glutaraldehyde: 0.0% using EHPI (Enhanced HPI) with 4% formaldehyde.

Target

  • Relevance
    L Glutamate is a non-essential amino acid naturally occurring in the L-form. It is the most common excitatory neurotransmitter in the Central Nervous System.
  • Alternative names
    • Glutamate antibody
    • L glutamate antibody

Images

  • IHC - Wholemount of mouse brain tissue labelling L Glutamate with ab62668. Sample was incubated with primary antibody (1/100 in 1X PBS + 0.3% Triton X-100 + 2 % normal goat serum) for 48 hours at 4°C. ab150174, a goat anti-chicken IgY Alexa Fluor® 555 (1/500) was used as the secondary antibody (assigned red color).

    See Abreview

References

ab62668 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
IHC - Wholemount
Sample
Mouse Tissue (Whole brain)
Specification
Whole brain

Abcam user community

Verified customer

Submitted Feb 17 2014

Question
Answer

Thanks for your call today and for your patience while I have looked into your question. I found that the antibody ab8893 does not react with free L-glutamate so I don't believe it will be suitable for the ELISA assay that you are planning. We do have a couple of L-glutamate antibodies, ab9440 and ab62668, that do not react with glutamate in peptides or proteins, so it will be specific for the free amino acid. However, we have not tested these antibodies in ELISA. We do have a testing discount program if you would like to try either of them in ELISA, and I would be happy to send more information about this program if you would like. Please let me know if you have any questions, or if there is anything else that we can do for you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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