Anti-L Glutamate antibody (ab9440)
Key features and details
- Rabbit polyclonal to L Glutamate
- Suitable for: IHC-FoFr, IHC-Fr, Immunomicroscopy, IHC-P
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-L Glutamate antibody
See all L Glutamate primary antibodies -
Description
Rabbit polyclonal to L Glutamate -
Host species
Rabbit -
Specificity
The antibody is calibrated against a spectrum of antigens to assure hapten selectivity. No measurable cross-reactivity (<1:1000) was detected against glutamate in peptides or proteins. Fixed tissue cross-reactivity was tested with known targets at the recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine, gamma-aminobutyrate, D/L-aspartate, agmatine, guanidine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Modest cross-reactivity (1:20) was detected against D-glutamate. Significant cross-reactivity (1:8) was detected against free NAAG in competition assays (NAAG is not retained in aldehyde-fixed tissue). -
Tested applications
Suitable for: IHC-FoFr, IHC-Fr, Immunomicroscopy, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
L-glutamate conjugated to glutaraldehyde
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General notes
The product is optimized for HPI/EHPI with gold or fluorescence detection using etched plastic sections. Filter diluted reagents with 0.2 mm syringe filters before use on EM grids. Enzyme-linked visualizations can be used but will compress the signal dynamic range and are less sensitive.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Thimerosal (merthiolate)
Constituents: Phosphate Buffer, 1% Goat Serum -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | (1) |
Use at an assay dependent concentration. Use with frozen or vibratome sections is possible but will not yield optimal images as IgGs penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effects occur.
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| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| Immunomicroscopy |
Use at an assay dependent concentration.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-FoFr
Use at an assay dependent concentration. Use with frozen or vibratome sections is possible but will not yield optimal images as IgGs penetrate aldehyde cross-linked tissue poorly and most amino acids are present at such high levels that prozone effects occur. |
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IHC-Fr
Use at an assay dependent concentration. |
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Immunomicroscopy
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
Target
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Relevance
L Glutamate is a non-essential amino acid naturally occurring in the L-form. It is the most common excitatory neurotransmitter in the Central Nervous System. -
Alternative names
- Glutamate antibody
- L glutamate antibody
Images
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Immunohistochemistry (Frozen sections) - Anti-L Glutamate antibody (ab9440)This image is courtesy of an Abreview submitted by Patricia Bogdanov
ab9440 staining L Gluramate in Mouse retina tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with methanol, permeabilized with 0.025% TBS-Triton X-100 and blocked with 5% serum for 1 hour. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 18 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab9440 has been referenced in 9 publications.
- Shivashankar G et al. Proinflammatory cytokines trigger biochemical and neurochemical changes in mouse retinal explants exposed to hyperglycemic conditions. Mol Vis 26:277-290 (2020). PubMed: 32300272
- Chang LY et al. Evidence of Synaptic and Neurochemical Remodeling in the Retina of Aging Degus. Front Neurosci 14:161 (2020). PubMed: 32256305
- Wu Z et al. Tonic inhibition in dentate gyrus impairs long-term potentiation and memory in an Alzhiemer's disease model. Nat Commun 5:4159 (2014). PubMed: 24923909
- Li D et al. Optogenetic activation of LiGluR-expressing astrocytes evokes anion channel-mediated glutamate release. J Physiol 590:855-73 (2012). PubMed: 22219341
- Friedrich T et al. Mutation of zebrafish dihydrolipoamide branched-chain transacylase E2 results in motor dysfunction and models maple syrup urine disease. Dis Model Mech 5:248-58 (2012). ICC/IF ; Zebrafish . PubMed: 22046030
- Wilkie IC et al. The elusive role of l-glutamate as an echinoderm neurotransmitter: evidence for its involvement in the control of crinoid arm muscles. Zoology (Jena) : (2012). PubMed: 23141629
- Wilkie IC et al. Physiological and immunocytochemical evidence that glutamatergic neurotransmission is involved in the activation of arm autotomy in the featherstar Antedon mediterranea (Echinodermata: Crinoidea). J Exp Biol 213:2104-15 (2010). IHC-R ; Starfish . PubMed: 20511525
- Hou S et al. Plasticity of lumbosacral propriospinal neurons is associated with the development of autonomic dysreflexia after thoracic spinal cord transection. J Comp Neurol 509:382-99 (2008). PubMed: 18512692
- Sun D et al. Metabolic and functional profiling of the normal rat retina. J Comp Neurol 505:92-113 (2007). IHC ; Rat . PubMed: 17729258