Overview

  • Product name

    L-Glutamate, excitatory neurotransmitter
  • Description

    Excitatory neurotransmitter
  • Biological description

    Major excitatory neurotransmitter. Acts at glutamate receptors. Active in vivo.
  • Purity

    > 99%
  • CAS Number

    56-86-0
  • Chemical structure

    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab120049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with L-Glutamate (ab120049), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of L-Glutamate, as described in literature.
    The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120049 (L-Glutamate) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:

  • Hainsworth AH  et al. Glutamate receptor-mediated inhibition of L-glutamate efflux from cerebral cortex in vitro. Brain Res 1114:36-40 (2006). Read more (PubMed: 16904087) »
  • Kew JN & Kemp JA Ionotropic and metabotropic glutamate receptor structure and pharmacology. Psychopharmacology (Berl) 179:4-29 (2005). Read more (PubMed: 15731895) »

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

To prepare the 1 gram vial in 1 eq. NaOH, you'll need to solubilize each 14.7 mg of the L-glutamate in 1 mL of 100 mM NaOH (due to batch-specific molecular weight of 147.13 g/mol). So, to prepare the full vial, you'll add 68.03 mL of 100 mM NaOH (1000 mg / (14.7 mg per 1 mL). This solution can be further diluted with water to the desired concentration.

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Question
Answer

The answer to this can be found on the biochemicals FAQ section of the PWS. https://www.abcam.com/index.html?pageconfig=resource&rid=14735#NaOH

If you look under the Solubilisation heading then “How do I make solutions using NaOH equivalents?” there is a good explanation.

Please let me know if I can be of further assistance.

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