Overview

  • Product name

    Anti-L-Kynurenine Hydrolase antibody
    See all L-Kynurenine Hydrolase primary antibodies
  • Description

    Rabbit polyclonal to L-Kynurenine Hydrolase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human L-Kynurenine Hydrolase aa 1-307.
    Database link: Q16719

  • Positive control

    • IHC-P: Human liver and placenta tissues. WB: A549, HeLa and HepG2 whole cell lysates. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab225916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/200 - 1/2000.
IHC-P 1/20 - 1/200.
WB 1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 35,52 kDa).

Target

  • Function

    Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.
  • Tissue specificity

    Expressed in all tissues tested (heart, brain placenta, lung, liver, skeletal muscle, kidney and pancreas). Highest levels found in placenta, liver and lung. Expressed in all brain regions.
  • Pathway

    Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1.
    Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.
  • Involvement in disease

    Note=Xanthurenic aciduria manifesting as massive urinary excretion of large amounts of kynurenine, 3-hydroxykynurenine and xanthurenic acid has been observed in an individual carrying a homozygous missense change in KYNU (PubMed:17334708). The urinary pattern in the patient suggests kynureninase deficiency and a block in the conversion of kynurenine and 3-hydroxykynurenine to anthranilate and 3-hydroxyanthranilate, respectively.
  • Sequence similarities

    Belongs to the kynureninase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • kynU antibody
    • KYNU_HUMAN antibody
    • kynureninase (L-kynurenine hydrolase) antibody
    • Kynureninase antibody
    • L-kynurenine hydrolase antibody
    see all

Images

  • All lanes : Anti-L-Kynurenine Hydrolase antibody (ab225916) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution

    Predicted band size: 35,52 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?

  • Paraffin-embedded human liver tissue stained for L-Kynurenine Hydrolase using ab225916 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human placenta tissue stained for L-Kynurenine Hydrolase using ab225916 at 1/100 dilution in immunohistochemical analysis.

  • L-Kynurenine Hydrolase was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 µg ab225916. For Western Blot analysis, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.

    Lane 1: Rabbit monoclonal IgG IP in HeLa whole cell lysate.

    Lane 2: ab225916 IP in HeLa whole cell lysate.

    Lane 3: HeLa whole cell lysate 20 µg (Input).

References

ab225916 has not yet been referenced specifically in any publications.

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