Key features and details
- Rabbit polyclonal to L-Kynurenine Hydrolase
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-L-Kynurenine Hydrolase antibody
See all L-Kynurenine Hydrolase primary antibodies
DescriptionRabbit polyclonal to L-Kynurenine Hydrolase
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human L-Kynurenine Hydrolase aa 1-307.
Database link: Q16719
- IHC-P: Human liver and placenta tissues. WB: A549, HeLa and HepG2 whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/200 - 1/2000.|
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 35,52 kDa).|
FunctionCatalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.
Tissue specificityExpressed in all tissues tested (heart, brain placenta, lung, liver, skeletal muscle, kidney and pancreas). Highest levels found in placenta, liver and lung. Expressed in all brain regions.
PathwayAmino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1.
Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.
Involvement in diseaseNote=Xanthurenic aciduria manifesting as massive urinary excretion of large amounts of kynurenine, 3-hydroxykynurenine and xanthurenic acid has been observed in an individual carrying a homozygous missense change in KYNU (PubMed:17334708). The urinary pattern in the patient suggests kynureninase deficiency and a block in the conversion of kynurenine and 3-hydroxykynurenine to anthranilate and 3-hydroxyanthranilate, respectively.
Sequence similaritiesBelongs to the kynureninase family.
- Information by UniProt
- kynU antibody
- KYNU_HUMAN antibody
- kynureninase (L-kynurenine hydrolase) antibody
All lanes : Anti-L-Kynurenine Hydrolase antibody (ab225916) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 35,52 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Paraffin-embedded human liver tissue stained for L-Kynurenine Hydrolase using ab225916 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human placenta tissue stained for L-Kynurenine Hydrolase using ab225916 at 1/100 dilution in immunohistochemical analysis.
L-Kynurenine Hydrolase was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 µg ab225916. For Western Blot analysis, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
Lane 1: Rabbit monoclonal IgG IP in HeLa whole cell lysate.
Lane 2: ab225916 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (Input).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225916 has not yet been referenced specifically in any publications.