Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Lysate, Urine
- Sensitivity: 0.02 mM
Product nameL-Lactate Assay Kit (Colorimetric)
See all L-Lactate kits
Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
Range0.02 mM - 10 mM
Assay time0h 30m
L-Lactate Assay Kit (Colorimetric) (ab65331) uses an assay protocol where lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a probe to produce a color (λmax = 450 nm).
The kit detects L(+)-Lactate in biological samples such as serum or plasma, cells, tissues, cell culture and fermentation media.
Lactate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader
L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present (see D-Lactate assay kits) but only at about 1-5% of the concentration of L(+)-Lactate.
L-Lactate assay kit ab65331 is our most popular L-Lactate assay kit (colorimetric 450nm, range 0.02 mM - 10 mM). Alternative L-Lactate assay kits offer different readout modes/wavelengths and sensitivity/range:
- L-Lactate assay ab65330: colorimetric 570 nm, fluorometric Ex/Em 535/587 nm, range 0.001 mM - 10 mM
- L-Lactate assay ab169557: fluorometric Ex/Em 535/587 nm, range 0.2 µM - 50 µM
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
How other researchers have used L-Lactate Assay Kit ab65331
This Lactate assay kit has been used in publications in a variety of sample types, including:
- Human: THP-1 cell lysates1, MDA-MB-231 and HepG2 cell culture lysates2, cell culture supernatant (HepG2, A549, Huh7, PC3, LN229, HeLa)3, brain tissue4
- Mouse: brown adipose tissue lysate5, thymic lymphoma tissue6, cell culture supernatant7, T cell primary cell culture supernatants8, serum9, serum and muscle10
- Bovine: cumulus cell culture supernatant11
References: 1 - Tran UT and Kitami T 2019; 2 - Cui J et al 2019; 3 - Rodriguez ML et al 2018, Chen Y et al 2018, Zhang D et al 2018, Caino MC et al 2017, Birkenmeier K et al 2015; 4 - Sullivan RC et al 2019; 5 - Jeong JH et al 2018; 6 - Vara-Ciruelos D et al 2019; 7 - Fiorenzano et al 2016; 8 - Menk AV et al 2018; 9 - Deng W et al 2019, Guglielmetti C et al 2017, Kang R et al 2016; 10 - Kim HY et al 2016; 11 - Sinha et al 2017
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests L(+)-Lactate Standard (100 nmol/µl) Yellow 1 x 100µl Lactate Assay Buffer WM 1 x 25ml Lactate Enzyme Mix (lyophilized) Green 1 vial Lactate Substrate Mix Red 1 vial
RelevanceLactate (CH3CH(OH)COO-) plays important roles in many biological processes. Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance beween NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH).
Plasma lactate concentrations were determined using L-Lactate assay kit (ab65331) in Ark2C+/+ and Ark2C−/− (Arkadia-like gene) mice.
Linearity of dilution: concentration of L-Lactate in differently diluted (X-axis) biological samples, demonstrating a linearity of 89%-111% (concentrations corrected for by factor of dilution; duplicates; +/- SD).
Relative signal (RFU) in unfiltered human plasma (dilution 1:8), comparing L-lactate signals with background reading (no enzyme) after 10 minutes of incubation (duplicates +/- SD).
Standard curve with background signal subtracted (duplicates; +/- SD).
Lactate Standard Curve. The assay is performed following the kit (ab65331) protocol.
ab65331 has been referenced in 107 publications.
- Gowayed MA et al. Enhanced mitochondrial biogenesis is associated with the ameliorative action of creatine supplementation in rat soleus and cardiac muscles. Exp Ther Med 19:384-392 (2020). PubMed: 31853315
- Lai HT et al. GATA6 enhances the stemness of human colon cancer cells by creating a metabolic symbiosis through upregulating LRH-1 expression. Mol Oncol 14:1327-1347 (2020). PubMed: 32037723
- Grassi ES et al. Hypoxia-induced release, nuclear translocation, and signaling activity of a DLK1 intracellular fragment in glioma. Oncogene 39:4028-4044 (2020). PubMed: 32205867
- Chen WR et al. Melatonin Attenuates Calcium Deposition from Vascular Smooth Muscle Cells by Activating Mitochondrial Fusion and Mitophagy via an AMPK/OPA1 Signaling Pathway. Oxid Med Cell Longev 2020:5298483 (2020). PubMed: 32377301
- Timper K et al. GLP-1 Receptor Signaling in Astrocytes Regulates Fatty Acid Oxidation, Mitochondrial Integrity, and Function. Cell Metab 31:1189-1205.e13 (2020). PubMed: 32433922