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we've been trying to assay extracellular lactate (in the medium) conditioned by Drosophila cells. We use Schneider's medium + 10% FBS. We've used your ab65331 kit and measured the medium with no cells, and then medium conditioned for 24hrs from cells. The medium is put over 10kDa spin columns (Ab93349) to rid it of HMW factors. However, the results indicate that there is quite a bit of lactate in the medium in the absence of cells (the wells were treated the same way as the medium that was taken from the cell cultures). I cannot find any mention of lactate or pyruvate in the Schneider medium formulation. Can you help me understand where the lactate is coming from?
Asked on Jul 01 2013
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Fetal Bovin Serum contains LDH and will create high background levels. Using a low percent FBS (0.25-1%) or serum free media will reduce this background.
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Heather AllenAbcam Scientific Support
Answered on Jul 01 2013