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I have a question about this kit. Can't one just measure the increase in NADH produced at by measuring increased absorbance at OD340? Doesn't this correspond directly to lactate levels? Why is it necessary to measure production of formazon at OD450 intead?
Asked on Dec 10 2013
Yes, theoretically it can be done by monitoring the changes in NADH absorption at 340 nm.
However, measuring at this OD requires expensive quartz microplates/cuvettes.
Enzymatic assays like ab65331 are a better way to get more accurate measurements because the signal is more specific and magnified, allowing better sensitivity and accuracy.
Jeremy KasanovAbcam Scientific Support
Answered on Dec 10 2013