• Product name
    L-Lactate Assay Kit (Colorimetric/Fluorometric)
    See all L-Lactate kits
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate
  • Assay type
  • Sensitivity
    > 0.001 mM
  • Range
    0.001 mM - 10 mM
  • Assay time
    0h 40m
  • Species reactivity
    Reacts with: Mammals
  • Product overview

    L-Lactate Assay Kit (Colorimetric/Fluorometric) (ab65330) is an assay where lactate specifically reacts with a enzyme mix to generate a product, which interacts with lactate probe to produce color (570 nm) and fluorescence (at Ex/Em = 535/587 nm). The kit provides a convenient means for detecting L(+)-Lactate in biological samples such as in blood circulation, in cells, in culture mediums, in fermentation mediums, etc. There is no need for pretreatment or purification of samples.

    Visit our FAQs page for tips and troubleshooting.

    Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

  • Notes

    L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate.


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    L(+)-Lactate Standard (100 nmol/µl) Yellow 1 x 100µl
    Lactate Assay Buffer WM 1 x 25ml
    Lactate Enzyme Mix (lyophilized) Green 1 vial
    Lactate Probe (in DMSO, anhydrous) Red 1 x 200µl
  • Research areas
  • Relevance
    Lactate (CH3CH(OH)COO-) plays important roles in many biological processes. Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance beween NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH).


  • HaCaT cells are grown in normoxia for 3 days after infection and medium was tested to determine lactate concentration using L-Lactate assay kit (ab65330). HaCaT cells were infected with AdGFP-18E2 or AdGFP, transfected with Ctrl or HIF-1α siRNA.

  • Relative signal (RFU) in unfiltered human plasma (dilution 1:200) and mouse urine (dilution 1:200), comparing L-lactate signals with background readings ((-); no enzyme) after 10 minutes of incubation (duplicates +/- SD).

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Standard curve: mean of duplicates (+/- SD) with background reads subtracted

  • Lactate measured in cell culture supernatants showing quantity (µmol) per mL of tested sample. Samples were diluted 40-160 fold and measured fluorometrically.

  • Lactate measured in biological fluids showing quantity (µmol) per mL of tested sample. Samples were diluted 10-40 fold and measured fluorometrically.



This product has been referenced in:
  • Girardot T  et al. Intra-cellular lactate concentration in T lymphocytes from septic shock patients - a pilot study. Intensive Care Med Exp 6:5 (2018). Read more (PubMed: 29404815) »
  • Oh AR  et al. ChREBP deficiency leads to diarrhea-predominant irritable bowel syndrome. Metabolism N/A:N/A (2018). Read more (PubMed: 29669261) »

See all 11 Publications for this product

Customer reviews and Q&As

I can confirm that we suggest to start with 10mg of tissue (muscle for example) or 10 million cells if using single cells.

This will vary widely depending on the tissue or cell type.

If you are using a cell type where the ...

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I can confirm that the assay buffer and the standard need to be stored at -20C as stated on the datasheet.

However, if assays will be run in small batches, we can recommend aliquoting these components and storing them to prevent free...

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Yes, our ab65330 L-Lactate Assay Kit (Fluorometric) can be used for colorimetric detection. The difference this kit has with the ab65331 L-Lactate Assay Kit (Colorimetric) is indeed mainly the probe, but the detection limits are also different for...

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I cannot give the exact compositions of the enzyme mixes in these kits, but the probes are just pure probes in a solvent. They do not have any other components. The enzyme mixes are obviously a combination of multiple enzymes. It does not have any flor...

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Thank you for contacting us. It should have mentioned in the protocol the incubation time is 30 minutes. We will be sure to correct the protocol.

I hope this information is helpful to you. Please do not hesitate to contact us if you need ...

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Thank you for contacting Abcam.
Since the lactate dehydrogenase (LDH) in tissue lysates degrades lactate, be sure to keep your samples at -80ºC. Below is our recommended homogenization protocol:
1. Homogenize the tissue samples (should contain ...

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Thank you for your enquiry.

We would recommend to use the assay buffer provided with ab65330 L-Lactate Assay Kit (Fluorometric) for cells. This buffer contains some proprietary ingredients which are crucial for the working efficiency of this...

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Thank you for contacting Abcam earlier today.

To prepare an intracellular lysate to use in the assay, we recommend using at least 2 x 10^6 cells. Then homogenize the cells in the assay buffer that is provided and centrifuge it gently to get ri...

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Gracias por contactarnos.

El kit de detección de Lactato debe mantenerse a -20C. Almacenado a esta temperatura y evitando ciclos de congelación y descongelación, el kit dura hasta la fecha indicada en la caja del kit, que...

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Thank you for contacting us.

I am not familiar with the contents of MRSB media, but in general cell culture supernatants will be compatible with these kits. If the media contains serum, we recommend deproteinizing the samples using our 10 kDa...

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1-10 of 21 Abreviews or Q&A


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