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L-Lactate Assay Kit (Fluorometric, High Sensitivity) (ab169557)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1)Q&A (1)

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Functional Assay: Functional Assay: Picoprobe L-Lactate Assay Kit (ab169557)
  • Functional Assay: Functional Assay: Picoprobe L-Lactate Assay Kit (ab169557)
  • Lactate Levels in Various Samples
  • Functional Assay: Functional Assay: Picoprobe L-Lactate Assay Kit (ab169557)

Key features and details

  • Assay type: Quantitative
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Sample type: Adherent cells, Other biological fluids, Plasma, Serum, Suspension cells, Tissue
  • Sensitivity: 0.2 µM

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Overview

  • Product name

    L-Lactate Assay Kit (Fluorometric, High Sensitivity)
    See all L-Lactate kits
  • Detection method

    Fluorescent
  • Sample type

    Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Sensitivity

    < 0.2 µM
  • Range

    1000 nmol/well - 250000 nmol/well
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    High Sensitivity L-Lactate Assay Kit (ab169557) is suitable for measuring very low levels of L(+)-lactate in a variety of samples.


    In this assay, L(+)-lactate is specifically oxidized to form an intermediate that reacts with a colorless probe to generate fluorescence (Ex/Em = 535/587 nm), which is directly proportional to the amount of lactate.


    This simple, rapid and high-throughput suitable assay kit is the most sensitive lactate assay kit on the market. It can detect L(+)-lactate less than 0.2 µM in a variety of biological samples.

  • Notes

    L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present (see D-Lactate assay kits) but only at about 1-5% of the concentration of L(+)-Lactate.

     

    Alternative L-Lactate assay kits offer different readout modes/wavelengths and sensitivity/range:
    - this kit L-Lactate assay ab169557: fluorometric Ex/Em 535/587 nm, range 0.2 µM - 50 µM
    - our most popular L-Lactate assay ab65331: colorimetric 450nm, range 0.02 mM - 10 mM
    - L-Lactate assay ab65330: colorimetric 570 nm, fluorometric Ex/Em 535/587 nm, range 0.001 mM - 10 mM

    Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    L(+)-Lactate Standard (100mM) Yellow 1 x 100µl
    Lactate Assay Buffer WM 1 x 25ml
    Lactate Enzyme Mix (lyophilized) Green 1 vial
    Lactate Substrate Mix Red 1 vial
    PicoProbe Blue 1 x 0.4ml
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Sugar Assays
  • Relevance

    Lactate (CH3CH(OH)COO-) plays important roles in many biological processes. Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance beween NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH).

Associated products

    Images

    • Functional Assay: Functional Assay: Picoprobe L-Lactate Assay Kit (ab169557)
      Functional Assay: Functional Assay: Picoprobe L-Lactate Assay Kit (ab169557)

      Relative signal (RFU) in filtered mouse heart lysate (3.7 mg protein/mL prior to filtration) in different volumes, comparing L-Lactate signals (black) with background reading (white, no enzyme).

    • Functional Assay: Functional Assay: Picoprobe L-Lactate Assay Kit (ab169557)
      Functional Assay: Functional Assay: Picoprobe L-Lactate Assay Kit (ab169557)

      L-Lactate measured in unfiltered human plasma (dilution range 1:30-1:90) and mouse serum (dilution range 1:2500-1:7500); duplicates +/- SD. The background control levels (no enzyme) were below level of detection.

    • Lactate Levels in Various Samples
      Lactate Levels in Various Samples
      Measurement of Lactate levels in rat liver (1.2 µg), kidney (0.7 µg), muscle (0.45 µg) and in human serum (0.5 µL from 1:10 diluted serum). Assays were performed following kit protocol.
    • Functional Assay: Functional Assay: Picoprobe L-Lactate Assay Kit (ab169557)
      Functional Assay: Functional Assay: Picoprobe L-Lactate Assay Kit (ab169557)

      Standard curve with background signal subtracted (duplicates; +/- SD).

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab169557? Please let us know so that we can cite the reference in this datasheet.

    ab169557 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Lactate assay on HK-2 cells

    Average Good 4/5 (Ease of Use)
    Abreviews
    Abreviews
    I've used this kit to measure Lactate levels in both cells and supernatant.
    Cells:
    0.5*10^6 cells were sonicated in assay buffer. 10ul from the lysate were loaded on assay wells. readings were about 10 times higher than the highest value of standard curve. further dilution is needed.
    Medium:
    10ul were loaded directly to assay well (without de-proteinization).
    again, values were much higher than standard curve assay and had to be diluted.

    Over all, kit was fairly OK and produced reasonable data.

    Dr. Liad Hinden

    Verified customer

    Submitted Apr 06 2016

    Question

    section 6B part 2 of the protocol, "sample preparation" suggests using percholic acid/KOH protocol - can you please send me the detailed protocol for this step?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 17 2015

    Answer

    Please click the link below for detailed protocol

    https://docs.abcam.com/pdf/cancer/deproteinization_protocol.pdf

    Read More

    Padamjeet Singh

    Abcam Scientific Support

    Answered on Nov 17 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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