L-Lactate Assay Kit (Fluorometric, High Sensitivity) (ab169557)
- Datasheet
- References
- Protocols
Overview
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Product name
L-Lactate Assay Kit (Fluorometric, High Sensitivity)
See all L-Lactate kits -
Detection method
Fluorescent -
Sample type
Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
< 0.2 µM -
Range
1000 nmol/well - 250000 nmol/well -
Species reactivity
Reacts with: Other species, Mammals -
Product overview
High Sensitivity L-Lactate Assay Kit (ab169557) is suitable for measuring very low levels of L(+)-lactate in a variety of samples.
In this assay, L(+)-lactate is specifically oxidized to form an intermediate that reacts with a colorless probe to generate fluorescence (Ex/Em = 535/587 nm), which is directly proportional to the amount of lactate.
This simple, rapid and high-throughput suitable assay kit is the most sensitive lactate assay kit on the market. It can detect L(+)-lactate less than 0.2 µM in a variety of biological samples.
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Notes
L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present (see D-Lactate assay kits) but only at about 1-5% of the concentration of L(+)-Lactate.
Alternative L-Lactate assay kits offer different readout modes/wavelengths and sensitivity/range:
- this kit L-Lactate assay ab169557: fluorometric Ex/Em 535/587 nm, range 0.2 µM - 50 µM
- our most popular L-Lactate assay ab65331: colorimetric 450nm, range 0.02 mM - 10 mM
- L-Lactate assay ab65330: colorimetric 570 nm, fluorometric Ex/Em 535/587 nm, range 0.001 mM - 10 mM
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests L(+)-Lactate Standard (100mM) Yellow 1 x 100µl Lactate Assay Buffer WM 1 x 25ml Lactate Enzyme Mix (lyophilized) Green 1 vial Lactate Substrate Mix Red 1 vial PicoProbe Blue 1 x 0.4ml -
Research areas
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Relevance
Lactate (CH3CH(OH)COO-) plays important roles in many biological processes. Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance beween NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH).
Images
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Functional Assay: Functional Assay: Picoprobe L-Lactate Assay Kit (ab169557)Relative signal (RFU) in filtered mouse heart lysate (3.7 mg protein/mL prior to filtration) in different volumes, comparing L-Lactate signals (black) with background reading (white, no enzyme).
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Functional Assay: Functional Assay: Picoprobe L-Lactate Assay Kit (ab169557)L-Lactate measured in unfiltered human plasma (dilution range 1:30-1:90) and mouse serum (dilution range 1:2500-1:7500); duplicates +/- SD. The background control levels (no enzyme) were below level of detection.
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Lactate Levels in Various SamplesMeasurement of Lactate levels in rat liver (1.2 µg), kidney (0.7 µg), muscle (0.45 µg) and in human serum (0.5 µL from 1:10 diluted serum). Assays were performed following kit protocol. -
Functional Assay: Functional Assay: Picoprobe L-Lactate Assay Kit (ab169557)Standard curve with background signal subtracted (duplicates; +/- SD).
Protocols
References
ab169557 has not yet been referenced specifically in any publications.
