L-Leucine 7-amido-4-methylcoumarin, Fluorogenic substrate (ab145941)

Overview

  • Product name

    L-Leucine 7-amido-4-methylcoumarin, Fluorogenic substrate
  • Description

    Sensitive fluorogenic substrate for leucine aminopeptidase and aminopeptidase M
  • Alternative names

    • H-Leu-AMC
    • L-Leu-AMC
  • Biological description

    Sensitive fluorogenic substrate for leucine aminopeptidase and aminopeptidase M. The release of fluorescent 7-amino-4-methylcoumarin can be measured. Hydrolysis rates can be measured using either a continuous or stopped assay.

  • Purity

    > 98%
  • CAS Number

    66447-31-2
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    380nm
  • Emission

    460nm
  • Molecular weight

    288.37
  • Molecular formula

    C16H20N2O3
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Robinson MW  et al. MHJ_0125 is an M42 glutamyl aminopeptidase that moonlights as a multifunctional adhesin on the surface of Mycoplasma hyopneumoniae. Open Biol 3:130017 (2013). Read more (PubMed: 23594879) »

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