Overview

  • Product name
  • Description
    Rabbit polyclonal to l-Myc
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: LERAVSDRLA PGAPRGNPPK ASAAPDCTPS LEAGNPAPAA PCPLGEPKTQ, corresponding to internal sequence amino acids 105-154 of Human l-Myc

  • Positive control
    • Human foetal kidney lysate, human kidney tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab28739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 2.5 µg/ml. Predicted molecular weight: 40,25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Immunohistochemical staining of paraffin embedded human kidney with ab28739 at 4-8 ug/ml. Arrows indicate postive staining of epithelial cells of the renal tube.
  • Anti-l-Myc antibody (ab28739) at 2.5 µg/ml + Human foetal kidney lysate

    Secondary
    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size: 40,25 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?

References

ab28739 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for your reply. For the c-myc antibody, I suggest that you look at ab56 or ab11917. A positive control would be ab7914. Please let me know which would be best for you. I also hope that you are able to find the replacement l-myc that was sent and are able to run successful experiments with it. Let me know if you have any questions or concerns.

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Answer

Thank you for contacting Abcam.

I am sorry to hear of the continued disappointing results from ab28739. I would be glad to send you a different antibody against l-myc or to issue you a credit note for the purchase. Please let me know what you decide.

If you have any questions do not hesitate to contact me.

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Answer

To my knowledge, customized protocols were not used in for this product. I've linked our standard western blotting protocol here: https://www.abcam.com/index.html?pageconfig=resource&rid=13045 In our published sample, Anti-l-Myc antibody (ab28739)was used at 2.5 µg/ml. With a secondary HRP conjugated anti-Rabbit IgG used at 1: 50,000 - 100,000. However, because your positve controls have also failed, and since I have spoken with you about your protocols, I believe that the issue lies in the product itself. I would like to send you a free of charge replacement for ab28739 Anti-l-Myc antibody. Due to the holiday season, I would like to make sure that if I sent a replacement someone would be around to recieve it. Would it be alright to send this week (we have 1 in the U.S now), or would it be better if we held off the delivery until after the New Year? I look forward to your reply.

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Question
Answer

Thank you for contacting us regarding this product. I am sorry that you have been experiencing difficulties using our product and look forward to hearing the results of your positive control experiment. At Abcam, providing quality service is just as important as providing quality products. Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund. The Abcam Abpromise® guarantee: • 100% Scientific and Customer Support for any product you buy from Abcam or one of our authorized distributors. • We guarantee our products work in the tested species and applications stated on the datasheet. • We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase. • We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards. Please note these conditions to our Abpromise®: • Protocol information must be provided in order for the claim to be validated. • Antibodies tested in recombinant samples only are not guaranteed for use on endogenous samples. • “Predicted to react” information on the datasheets is provided for reference only and these species are not guaranteed. • Fast Track antibodies are covered for ELISA against the immunizing peptide only. I hope this information is helpful, but please do not hesitate to contact us with further questions.    

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 cell)
Loading amount
50 µg
Specification
HEK293 cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

Abcam user community

Verified customer

Submitted Aug 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Renal Pelvis of transgenic mouse)
Specification
Renal Pelvis of transgenic mouse
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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