Overview

  • Product name

    L1210 whole cell lysate
  • General notes

    Cell line: L1210 (lymphocytic leukemia).
    Growth media: DMEM and 10% horse serum.


    Mouse L1210 cell lysate was prepared by homogenization in modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.80
    Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notes

    Mouse L1210 cell lysate was prepared by homogenization in modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • Research areas

  • Background

    L1210 cells are derived from a 8month old DBA/2 female mouse ascitic fluid and are lymphoblast-like in morphology. The line has been extensively used in cytotoxicity assays of chemotherapeutic agents. It is also used for apoptosis and proliferation studies.

Applications

Our Abpromise guarantee covers the use of ab7184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. L1210 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

References

ab7184 has not yet been referenced specifically in any publications.

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