Key features and details
- Mouse monoclonal [M023] to L1CAM - Extracellular domain
- Suitable for: ICC/IF, WB, ELISA
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-L1CAM antibody [M023] - Extracellular domain
See all L1CAM primary antibodies
DescriptionMouse monoclonal [M023] to L1CAM - Extracellular domain
Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
Species reactivityReacts with: Human
Other Immunogen Type within Human L1CAM. The exact sequence is proprietary. Clone M023 was generated from a proprietary antigen related to the native human L1CAM expressed in the MeWo melanoma cell line
- WB: MeWo, MDA-MB-231, A431 and MCF7 cell lysate. Human brain tissue lysate. ICC/IF: MeWo cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab253290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 140 kDa.|
|ELISA||Use at an assay dependent concentration.|
FunctionCell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons.
Involvement in diseaseDefects in L1CAM are the cause of hydrocephalus due to stenosis of the aqueduct of Sylvius (HSAS) [MIM:307000]. Hydrocephalus is a condition in which abnormal accumulation of cerebrospinal fluid in the brain causes increased intracranial pressure inside the skull. This is usually due to blockage of cerebrospinal fluid outflow in the brain ventricles or in the subarachnoid space at the base of the brain. In children is typically characterized by enlargement of the head, prominence of the forehead, brain atrophy, mental deterioration, and convulsions. In adults the syndrome includes incontinence, imbalance, and dementia. HSAS is characterized by mental retardation and enlarged brain ventricles.
Defects in L1CAM are the cause of mental retardation-aphasia-shuffling gait-adducted thumbs syndrome (MASA) [MIM:303350]; also known as corpus callosum hypoplasia, psychomotor retardation, adducted thumbs, spastic paraparesis, and hydrocephalus or CRASH syndrome. MASA is an X-linked recessive syndrome with a highly variable clinical spectrum. Main clinical features include spasticity and hyperreflexia of lower limbs, shuffling gait, mental retardation, aphasia and adducted thumbs. The features of spasticity have been referred to as complicated spastic paraplegia type 1 (SPG1). Some patients manifest corpus callosum hypoplasia and hydrocephalus. Inter- and intrafamilial variability is very wide, such that patients with hydrocephalus, MASA, SPG1, and agenesis of corpus callosum can be present within the same family.
Defects in L1CAM are the cause of spastic paraplegia X-linked type 1 (SPG1) [MIM:303350]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Note=Defects in L1CAM may contribute to Hirschsprung disease by modifying the effects of Hirschsprung disease-associated genes to cause intestinal aganglionosis.
Defects in L1CAM are a cause of partial agenesis of the corpus callosum (ACCPX) [MIM:304100]. A syndrome characterized by partial corpus callosum agenesis, hypoplasia of inferior vermis and cerebellum, mental retardation, seizures and spasticity. Other features include microcephaly, unusual facies, and Hirschsprung disease in some patients.
Sequence similaritiesBelongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
Contains 5 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localizationCell membrane.
- Information by UniProt
- Antigen identified by monoclonal antibody R1 antibody
- CAML1 antibody
- CD171 antibody
Representative Standard Curve using mouse monoclonal anti-L1CAM for ELISA capture of human recombinant L1CAM protein with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.
Paraformaldehyde-fixed MeWo (Human malignant melanoma cell line) cells labeling L1CAM using ab253290 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.
All lanes : Anti-L1CAM antibody [M023] - Extracellular domain (ab253290) at 1/1000 dilution
Lane 1 : MDA-MB-231 (Human cell line) cell lysate
Lane 2 : MeWo (Human malignant melanoma cell line) cell lysate
Lane 3 : Human brain tissue lysate
Lane 4 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
Predicted band size: 140 kDa
Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hour at room temperature.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab253290 has not yet been referenced specifically in any publications.