Key features and details
- Rabbit polyclonal to L2HGDH
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-L2HGDH antibody
See all L2HGDH primary antibodies
DescriptionRabbit polyclonal to L2HGDH
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Orangutan
Recombinant fragment corresponding to Human L2HGDH aa 1-220.
MVPALRYLVGACGRARGLFAGGSPGACGFASGRPRPLCGGSRSASTSSFD IVIVGGGIVGLASARALILRHPSLSIGVLEKEKDLAVHQTGHNSGVIHSG IYYKPESLKAKLCVQGAALLYEYCQQKGISYKQCGKLIVAVEQEEIPRLQ ALYEKGLQNGVPGLRLIQQEDIKKKEPYCRGLMAIDCPHTGIVDYRQVAL SFAQDFQEAGGSVLTNFEVK
Database link: Q9H9P8
- WB: Mouse heart lysate. IHC-P: Human testis tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).|
|IHC-P||1/20 - 1/200.|
Tissue specificityWidely expressed. Highly expressed in brain, testis and muscle. Expressed to a lower extent in lymphocytes, fibroblasts, keratinocytes, placenta, bladder, small intestine, liver and bone marrow.
Involvement in diseaseDefects in L2HGDH are the cause of L-2-hydroxyglutaric aciduria (L2HGA) [MIM:236792]. L2HGA is a rare autosomal recessive disorder clinically characterized by mild psychomotor delay in the first years of life, followed by progressive cerebellar ataxia, dysarthria and moderate to severe mental retardation. Diagnosis is based on the presence of an excess of L-2-hydroxyglutaric acid in urine, blood and cerebrospinal fluid.
Sequence similaritiesBelongs to the L2HGDH family.
- Information by UniProt
- 2 hydroxyglutarate dehydrogenase antibody
- Alpha hydroxyglutarate oxidoreductase antibody
- Alpha ketoglutarate reductase antibody
Anti-L2HGDH antibody (ab230230) at 1/1000 dilution + Mouse heart tissue lysate
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Paraffin-embedded human testis tissue stained for L2HGDH using ab230230 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230230 has not yet been referenced specifically in any publications.