Overview

  • Product name

    Anti-L2/HNK-1 Carbohydrate antibody [ZN-12]
  • Description

    Mouse Monoclonal [ZN-12] to L2/HNK-1 Carbohydrate
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Human, Zebrafish
  • Immunogen

    Other Immunogen Type. This information is considered to be commercially sensitive.

  • Positive control

    • SH-SY5Y cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99.9% HEPES buffered saline
  • Concentration information loading...
  • Purity

    SDS-PAGE
  • Purification notes

    ab174437 is produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. Near homogeneity as judged by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    ZN-12
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500.
ICC/IF Use a concentration of 5 µg/ml.

Images

  • ab174437 staining L2/HNK-1 Carbohydrate in 4 dpf zebrafish retina sections by IHC-Fr. The tissue was fixed with paraformaldehyde and an antigen retrieval step was performed with sodium citrate pH 6. Blocking of the sample was done with 5% BSA in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab174437 at 1/500 in blocking solution for 16h at 4°C. An alexa 647 conjugated goat anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI.  ab174437 stains the inner nuclear layer and inner plexiform layer (red). 

    See Abreview

  • Immunocytochemistry with SH-SY5Y cells stained with ab174437 in red and DAPI in blue, as a counter stain. Fixation: Paraformaldehyde fixed (4%, 20 min) Permeabilization: Triton X-100 permeabilized (0.1%, 10 min) Blocking: 10% Goat Serum/PBS (1 hour) Primary: ab174437  5 µg/mL (10% Goat Serum/PBS, 2hr at room temperature or overnight at 4°C). Secondary: Alexa 594 goat anti-mouse IgG1 used at a 1/1000 dilution in 10% Goat Serum/PBS for 1hr at RT. Washing: 3x 1% Goat Serum/PBS, 10 minutes/wash DAPI: 2 µM in 1% Goat Serum/PBS, 10 minutes.

References

ab174437 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Filter by Species

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Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 23°C
Sample
Zebrafish Tissue sections (Zebrafish retina)
Specification
Zebrafish retina
Permeabilization
No
Fixative
Paraformaldehyde

Dr. Ryan Macdonald

Verified customer

Submitted Aug 02 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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