Anti-L3MBTL2 antibody (ab101272)
Key features and details
- Rabbit polyclonal to L3MBTL2
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-L3MBTL2 antibody
See all L3MBTL2 primary antibodies -
Description
Rabbit polyclonal to L3MBTL2 -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanIP Human -
Immunogen
Synthetic peptide corresponding to a region between residue 655 and 705 of Human L3MBTL2 (NP_113676.2).
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Positive control
- HeLa whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab101272 was affinity purified using an epitope specific to L3MBTL2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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IP |
Human
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All applications |
Cow
Chimpanzee
Gorilla
Orangutan
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Application | Abreviews | Notes |
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated 'Lys-20' on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on 'Lys-4', 'Lys-9' or 'Lys-27'. -
Sequence similarities
Contains 1 FCS-type zinc finger.
Contains 4 MBT repeats. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 513297 Cow
- Entrez Gene: 83746 Human
- Entrez Gene: 100173412 Orangutan
- Omim: 611865 Human
- SwissProt: Q1JQD9 Cow
- SwissProt: Q969R5 Human
- SwissProt: Q5R737 Orangutan
- Unigene: 517641 Human
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Alternative names
- 3bt like 2 protein antibody
- 3bt like protein antibody
- DKFZP761I141 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L3MBTL2 antibody (ab101272)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling L3MBTL2 with ab101272 at 1/1000 (1µg/ml).
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Detection of L3MBTL2 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab101272 at 3 µg/mg lysate for IP (Lane 1). An anti-L3MBTL2 antibody which recognizes an upstream epitope was used in Lane 2, and also for the subsequent WB detection at 1 µg/ml. Lane 3 represents control IgG. Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab101272 has not yet been referenced specifically in any publications.