Anti-L3MBTL2 antibody (ab101272)

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Overview

  • Product name

    Anti-L3MBTL2 antibody
    See all L3MBTL2 primary antibodies

  • Description

    Rabbit polyclonal to L3MBTL2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee, Gorilla, Orangutan

  • Immunogen

    Synthetic peptide corresponding to a region between residue 655 and 705 of Human L3MBTL2 (NP_113676.2).

  • Positive control

    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab101272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Function

      Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated 'Lys-20' on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on 'Lys-4', 'Lys-9' or 'Lys-27'.

    • Sequence similarities

      Contains 1 FCS-type zinc finger.
      Contains 4 MBT repeats.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • 3bt like 2 protein antibody
        • 3bt like protein antibody
        • DKFZP761I141 antibody
        • H-l(3)mbt-like protein 2 antibody
        • H-l(3)mbt-like protein antibody
        • Hl antibody
        • Hl(3)mbt like protein antibody
        • Hl(3)mbtl antibody
        • L antibody
        • l(3)mbt like 2 (Drosophila) antibody
        • l(3)mbt like 2 antibody
        • L(3)mbt-like 2 protein antibody
        • L(3)mbt-like protein 2 antibody
        • L3MBTL2 antibody
        • Lethal antibody
        • Lethal(3)malignant brain tumor-like 2 protein antibody
        • Lethal(3)malignant brain tumor-like protein 2 antibody
        • LMBL2_HUMAN antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L3MBTL2 antibody (ab101272)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L3MBTL2 antibody (ab101272)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling L3MBTL2 with ab101272 at 1/1000 (1µg/ml).
      • Immunoprecipitation - Anti-L3MBTL2 antibody (ab101272)
        Immunoprecipitation - Anti-L3MBTL2 antibody (ab101272)
        Detection of L3MBTL2 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab101272 at 3 µg/mg lysate for IP (Lane 1). An anti-L3MBTL2 antibody which recognizes an upstream epitope was used in Lane 2, and also for the subsequent WB detection at 1 µg/ml. Lane 3 represents control IgG. Detection: Chemiluminescence with an exposure time of 30 seconds.

      References

      ab101272 has not yet been referenced specifically in any publications.

      Publishing research using ab101272? Please let us know so that we can cite the reference in this datasheet.

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