Anti-L3MBTL2 antibody (ab101272)
Key features and details
- Rabbit polyclonal to L3MBTL2
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-L3MBTL2 antibody
See all L3MBTL2 primary antibodies -
Description
Rabbit polyclonal to L3MBTL2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to a region between residue 655 and 705 of Human L3MBTL2 (NP_113676.2).
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Positive control
- HeLa whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab101272 was affinity purified using an epitope specific to L3MBTL2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated 'Lys-20' on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on 'Lys-4', 'Lys-9' or 'Lys-27'. -
Sequence similarities
Contains 1 FCS-type zinc finger.
Contains 4 MBT repeats. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 513297 Cow
- Entrez Gene: 83746 Human
- Entrez Gene: 100173412 Orangutan
- Omim: 611865 Human
- SwissProt: Q1JQD9 Cow
- SwissProt: Q969R5 Human
- SwissProt: Q5R737 Orangutan
- Unigene: 517641 Human
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Alternative names
- 3bt like 2 protein antibody
- 3bt like protein antibody
- DKFZP761I141 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling L3MBTL2 with ab101272 at 1/1000 (1µg/ml).
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Detection of L3MBTL2 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab101272 at 3 µg/mg lysate for IP (Lane 1). An anti-L3MBTL2 antibody which recognizes an upstream epitope was used in Lane 2, and also for the subsequent WB detection at 1 µg/ml. Lane 3 represents control IgG. Detection: Chemiluminescence with an exposure time of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab101272 has not yet been referenced specifically in any publications.