Overview

  • Product name
    Anti-LacI antibody [9A5]
  • Description
    Mouse monoclonal [9A5] to LacI
  • Host species
    Mouse
  • Specificity
    The clone number has been updated from (0.T.81) to (9A5) both clone numbers name the same antibody clone.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Escherichia coli
  • Immunogen

    Recombinant TrpE fusion protein containing full length E. coli LacI.

  • Positive control
    • Lac1 transfected Cos-1 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 30% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    9A5
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
IHC-FoFr
  • Application notes
    ICC/IF: Use at an assay dependent dilution.
    IHC-Fo: Use at an assay dependent dilution. The antibody shows nuclear immunostaining in Lac1 transgenic tissue fixed with 4% paraformaldehyde and permeabilized with Triton X100.
    WB: Use at a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Cellular localization
      Nuclear
    • Database links
      • Alternative names
        • DNA binding transcriptional repressor antibody
        • LacI antibody
        • Transcriptional repressor of the lac operon antibody

      Images

      • Ab33832 (1/1000) staining Lacl in Escherichia coli cultured cell lysate
        Secondary Antibody: An HRP-conjugated Donkey anti-mouse monoclonal (1/20000)

        Lane 1: Molecular weight Marker
        Lane 2: Rainbow Molecular weight Marker
        Lane 3: Blank
        Lane 4: pCMV Vector cell lysate 1
        Lane 5: pCMV Vector cell lysate 2

        Performed under reduced and denaturing running conditions.
        Blocking step: 5% Milk for 1 hour at 22°C.

        See Abreview

      References

      This product has been referenced in:
      • Russell CW & Mulvey MA The Extraintestinal Pathogenic Escherichia coli Factor RqlI Constrains the Genotoxic Effects of the RecQ-Like Helicase RqlH. PLoS Pathog 11:e1005317 (2015). Read more (PubMed: 26636713) »
      • Sienski G  et al. Silencio/CG9754 connects the Piwi-piRNA complex to the cellular heterochromatin machinery. Genes Dev 29:2258-71 (2015). Human . Read more (PubMed: 26494711) »
      See all 4 Publications for this product

      Customer reviews and Q&As

      1-2 of 2 Abreviews or Q&A

      Answer

      Thank you for contacting us. I am sorry to hear that the antibody does not seem to work as exptected. As we discussed, loading more protein (20-30 ug) might help to solve the problem. Should thisnot help, please let me know so that we can discuss other options of resolving this case (such as replacement, credit or refund). I wish you good luck with the experiments. In the meantime, please do not hesitate to contact us if you need any more advice or information.

      Read More
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Western blot
      Sample
      Escherichia coli Cell lysate - whole cell (Cultured cell lysate)
      Loading amount
      40 µg
      Specification
      Cultured cell lysate
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

      Abcam user community

      Verified customer

      Submitted Nov 27 2009

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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