Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)

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Western blot - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
  • Flow Cytometry - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
  • Immunocytochemistry - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
  • Immunoprecipitation - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
  • In-Cell ELISA - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)

Key features and details

  • Mouse monoclonal [98A-1F9BB1] to Lactate Dehydrogenase
  • Suitable for: WB, Flow Cyt, ICC, IP, In-Cell ELISA
  • Reacts with: Mouse, Rat, Rabbit, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Lactate Dehydrogenase antibody [98A-1F9BB1]
    See all Lactate Dehydrogenase primary antibodies

  • Description

    Mouse monoclonal [98A-1F9BB1] to Lactate Dehydrogenase

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC, IP, In-Cell ELISAmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Human
    Does not react with: Cow

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • Human, mouse and rat liver and heart homogenates; HDFn, HeLa, HepG2, H9C2, H4IIE and MEF whole cell lysates; Lactate Dehydrogenase purified from rabbit skeletal muscle; HeLa cells.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab135396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
ICC Use a concentration of 1 µg/ml.
IP Use a concentration of 1 µg/ml.
In-Cell ELISA Use a concentration of 1 µg/ml.

Target

  • Pathway

    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.

  • Involvement in disease

    Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.

  • Sequence similarities

    Belongs to the LDH/MDH superfamily. LDH family.

  • Post-translational
    modifications

    ISGylated.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Cell proliferation-inducing gene 19 protein antibody
    • GSD11 antibody
    • L lactate dehydrogenase B chain antibody
    • L-lactate dehydrogenase A chain antibody
    • Lactate dehydrogenase A antibody
    • Lactate dehydrogenase B antibody
    • Lactate dehydrogenase c variant 1 antibody
    • Lactate dehydrogenase c variant 3 antibody
    • Lactate dehydrogenase c variant 4 antibody
    • Lactate dehydrogenase C4 antibody
    • Lactate dehydrogenase H chain antibody
    • Lactate dehydrogenase M antibody
    • LDH A antibody
    • LDH B antibody
    • LDH H antibody
    • LDH heart subunit antibody
    • LDH M antibody
    • LDH muscle subunit antibody
    • LDH-A antibody
    • LDH-M antibody
    • LDH1 antibody
    • ldha antibody
    • LDHA_HUMAN antibody
    • LDHBD antibody
    • LDHM antibody
    • MS1111 antibody
    • PIG19 antibody
    • Proliferation inducing gene 19 antibody
    • Proliferation-inducing gene 19 antibody
    • Renal carcinoma antigen NY REN 46 antibody
    • Renal carcinoma antigen NY-REN-59 antibody
    • TRG 5 antibody
    • TRG5 antibody
    see all

Images

  • Western blot - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
    Western blot - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
    All lanes : Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396) at 1 µg/ml

    Lanes 1 & 8 : Ladder
    Lane 2 : Human liver homogenate at 15 µg
    Lane 3 : Human heart homogenate at 15 µg
    Lane 4 : Rat liver homogenate at 15 µg
    Lane 5 : Rat heart homogenate at 15 µg
    Lane 6 : Mouse liver homogenate at 15 µg
    Lane 7 : Mouse heart homogenate at 15 µg
    Lane 9 : Human fibroblast whole cell lysate at 15 µg
    Lane 10 : HeLa whole Cell Lysate at 15 µg
    Lane 11 : HepG2 whole cell lysate at 15 µg
    Lane 12 : H9C2 (Rat cardiomyoblast cell line) whole Cell Lysate at 15 µg
    Lane 13 : H4IIE (Rat hepatoma cell line) whole Cell Lysate at 15 µg
    Lane 14 : MEF (Mouse embryonic fibroblast cell line) whole Cell Lysate at 15 µg
    Lane 15 : LDH (purified from rabbit skeletal muscle) at 0.015 µg

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - AP at 1/3000 dilution

    Predicted band size: 37 kDa



    The high background signal in mouse tissue sample was caused by the direct reaction between the mouse IgG in mouse tissue preps and the goat anti-mouse secondary antibody. LDHA and LDHB are resolved in tissues containing both isoforms.
  • Flow Cytometry - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
    Flow Cytometry - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
    Flow cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml (blue) or an equal amount of an isotype control antibody (red). 1% BSA was used as the blocking reagent for all the blocking steps.
  • Immunocytochemistry - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
    Immunocytochemistry - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
    Immunocytochemistry analysis of Paraformaldehyde fixed Hela cells labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml. Detection utilised Alexa 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution.
  • Immunoprecipitation - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
    Immunoprecipitation - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
    Immunoprecipitation of Lactate Dehydrogenase from 1mg of Hela Cell Lysate with ab135396 at 1 µg/ml. The gel was stained with Coomassie brilliant blue G.
  • In-Cell ELISA - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
    In-Cell ELISA - Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396)
    In-cell ELISA analysis of paraformaldehyde/PBS fixed HeLa cells seeded at 20k cells/well. ab135396 was serially diluted in 1%BSA/PBS and detected with goat anti-mouse dye-conjugated antibody at 1/1000 in 1%BSA/PBS.

References (2)

Publishing research using ab135396? Please let us know so that we can cite the reference in this datasheet.

ab135396 has been referenced in 2 publications.

  • Jacobs RA  et al. Twenty-eight days of exposure to 3454 m increases mitochondrial volume density in human skeletal muscle. J Physiol 594:1151-66 (2016). PubMed: 26339730
  • Branco AF  et al. Gene Expression Profiling of H9c2 Myoblast Differentiation towards a Cardiac-Like Phenotype. PLoS One 10:e0129303 (2015). PubMed: 26121149

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