Product nameAnti-Lactate Dehydrogenase antibody [98A-1F9BB1]
See all Lactate Dehydrogenase primary antibodies
DescriptionMouse monoclonal [98A-1F9BB1] to Lactate Dehydrogenase
Tested applicationsSuitable for: WB, Flow Cyt, ICC, IP, In-Cell ELISAmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Human
Does not react with: Cow
Tissue, cells or virus. This information is considered to be commercially sensitive.
- Human, mouse and rat liver and heart homogenates; HDFn, HeLa, HepG2, H9C2, H4IIE and MEF whole cell lysates; Lactate Dehydrogenase purified from rabbit skeletal muscle; HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.357% HEPES, 0.877% Sodium chloride
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Our Abpromise guarantee covers the use of ab135396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC||Use a concentration of 1 µg/ml.|
|IP||Use a concentration of 1 µg/ml.|
|In-Cell ELISA||Use a concentration of 1 µg/ml.|
PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in diseaseDefects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.
- Information by UniProt
- Cell proliferation-inducing gene 19 protein antibody
- GSD11 antibody
- L lactate dehydrogenase B chain antibody
All lanes : Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396) at 1 µg/ml
Lanes 1 & 8 : Ladder
Lane 2 : Human liver homogenate at 15 µg
Lane 3 : Human heart homogenate at 15 µg
Lane 4 : Rat liver homogenate at 15 µg
Lane 5 : Rat heart homogenate at 15 µg
Lane 6 : Mouse liver homogenate at 15 µg
Lane 7 : Mouse heart homogenate at 15 µg
Lane 9 : Human fibroblast whole cell lysate at 15 µg
Lane 10 : HeLa whole Cell Lysate at 15 µg
Lane 11 : HepG2 whole cell lysate at 15 µg
Lane 12 : H9C2 (Rat cardiomyoblast cell line) whole Cell Lysate at 15 µg
Lane 13 : H4IIE (Rat hepatoma cell line) whole Cell Lysate at 15 µg
Lane 14 : MEF (Mouse embryonic fibroblast cell line) whole Cell Lysate at 15 µg
Lane 15 : LDH (purified from rabbit skeletal muscle) at 0.015 µg
All lanes : Goat polyclonal to Mouse IgG - AP at 1/3000 dilution
Predicted band size: 37 kDa
The high background signal in mouse tissue sample was caused by the direct reaction between the mouse IgG in mouse tissue preps and the goat anti-mouse secondary antibody. LDHA and LDHB are resolved in tissues containing both isoforms.
Flow cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml (blue) or an equal amount of an isotype control antibody (red). 1% BSA was used as the blocking reagent for all the blocking steps.
Immunocytochemistry analysis of Paraformaldehyde fixed Hela cells labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml. Detection utilised Alexa 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution.
Immunoprecipitation of Lactate Dehydrogenase from 1mg of Hela Cell Lysate with ab135396 at 1 µg/ml. The gel was stained with Coomassie brilliant blue G.
In-cell ELISA analysis of paraformaldehyde/PBS fixed HeLa cells seeded at 20k cells/well. ab135396 was serially diluted in 1%BSA/PBS and detected with goat anti-mouse dye-conjugated antibody at 1/1000 in 1%BSA/PBS.
This product has been referenced in:
- Jacobs RA et al. Twenty-eight days of exposure to 3454 m increases mitochondrial volume density in human skeletal muscle. J Physiol 594:1151-66 (2016). Read more (PubMed: 26339730) »
- Branco AF et al. Gene Expression Profiling of H9c2 Myoblast Differentiation towards a Cardiac-Like Phenotype. PLoS One 10:e0129303 (2015). Read more (PubMed: 26121149) »