Key features and details
- Mouse monoclonal [98A-1F9BB1] to Lactate Dehydrogenase
- Suitable for: WB, Flow Cyt, ICC, IP, In-Cell ELISA
- Reacts with: Mouse, Rat, Rabbit, Human
- Isotype: IgG1
Product nameAnti-Lactate Dehydrogenase antibody [98A-1F9BB1]
See all Lactate Dehydrogenase primary antibodies
DescriptionMouse monoclonal [98A-1F9BB1] to Lactate Dehydrogenase
Tested applicationsSuitable for: WB, Flow Cyt, ICC, IP, In-Cell ELISAmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Human
Does not react with: Cow
Tissue, cells or virus. This information is considered to be commercially sensitive.
- Human, mouse and rat liver and heart homogenates; HDFn, HeLa, HepG2, H9C2, H4IIE and MEF whole cell lysates; Lactate Dehydrogenase purified from rabbit skeletal muscle; HeLa cells.
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.877% Sodium chloride, 0.357% HEPES
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Our Abpromise guarantee covers the use of ab135396 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa.|
|Flow Cyt||Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC||Use a concentration of 1 µg/ml.|
|IP||Use a concentration of 1 µg/ml.|
|In-Cell ELISA||Use a concentration of 1 µg/ml.|
PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in diseaseDefects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.
- Information by UniProt
- Cell proliferation-inducing gene 19 protein antibody
- GSD11 antibody
- L lactate dehydrogenase B chain antibody
All lanes : Anti-Lactate Dehydrogenase antibody [98A-1F9BB1] (ab135396) at 1 µg/ml
Lanes 1 & 8 : Ladder
Lane 2 : Human liver homogenate at 15 µg
Lane 3 : Human heart homogenate at 15 µg
Lane 4 : Rat liver homogenate at 15 µg
Lane 5 : Rat heart homogenate at 15 µg
Lane 6 : Mouse liver homogenate at 15 µg
Lane 7 : Mouse heart homogenate at 15 µg
Lane 9 : Human fibroblast whole cell lysate at 15 µg
Lane 10 : HeLa whole Cell Lysate at 15 µg
Lane 11 : HepG2 whole cell lysate at 15 µg
Lane 12 : H9C2 (Rat cardiomyoblast cell line) whole Cell Lysate at 15 µg
Lane 13 : H4IIE (Rat hepatoma cell line) whole Cell Lysate at 15 µg
Lane 14 : MEF (Mouse embryonic fibroblast cell line) whole Cell Lysate at 15 µg
Lane 15 : LDH (purified from rabbit skeletal muscle) at 0.015 µg
All lanes : Goat polyclonal to Mouse IgG - AP at 1/3000 dilution
Predicted band size: 37 kDa
The high background signal in mouse tissue sample was caused by the direct reaction between the mouse IgG in mouse tissue preps and the goat anti-mouse secondary antibody. LDHA and LDHB are resolved in tissues containing both isoforms.
Flow cytometric analysis of HeLa cells (fixed and permeabilized with methanol) labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml (blue) or an equal amount of an isotype control antibody (red). 1% BSA was used as the blocking reagent for all the blocking steps.
Immunocytochemistry analysis of Paraformaldehyde fixed Hela cells labelling Lactate Dehydrogenase with ab135396 at 1 µg/ml. Detection utilised Alexa 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution.
Immunoprecipitation of Lactate Dehydrogenase from 1mg of Hela Cell Lysate with ab135396 at 1 µg/ml. The gel was stained with Coomassie brilliant blue G.
In-cell ELISA analysis of paraformaldehyde/PBS fixed HeLa cells seeded at 20k cells/well. ab135396 was serially diluted in 1%BSA/PBS and detected with goat anti-mouse dye-conjugated antibody at 1/1000 in 1%BSA/PBS.
ab135396 has been referenced in 2 publications.
- Jacobs RA et al. Twenty-eight days of exposure to 3454 m increases mitochondrial volume density in human skeletal muscle. J Physiol 594:1151-66 (2016). PubMed: 26339730
- Branco AF et al. Gene Expression Profiling of H9c2 Myoblast Differentiation towards a Cardiac-Like Phenotype. PLoS One 10:e0129303 (2015). PubMed: 26121149