Anti-Lactate Dehydrogenase antibody (ab191332)
Key features and details
- Goat polyclonal to Lactate Dehydrogenase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Lactate Dehydrogenase antibody
See all Lactate Dehydrogenase primary antibodies -
Description
Goat polyclonal to Lactate Dehydrogenase -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mammals -
Immunogen
Full length native protein (purified) corresponding to Rabbit Lactate Dehydrogenase aa 1 to the C-terminus. (Isolated from rabbit muscle).
Database link: P13491 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading...
-
Purity
IgG fraction -
Purification notes
ab191332 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
|
Notes |
---|
WB
1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa). |
Target
-
Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
Involvement in disease
Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. -
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 3939 Human
- Entrez Gene: 3945 Human
- Omim: 150000 Human
- Omim: 150100 Human
- SwissProt: P00338 Human
- SwissProt: P07195 Human
- SwissProt: P07864 Human
- Unigene: 2795 Human
-
Alternative names
- Cell proliferation-inducing gene 19 protein antibody
- GSD11 antibody
- L lactate dehydrogenase B chain antibody
see all
Images
-
All lanes : Anti-Lactate Dehydrogenase antibody (ab191332) at 1/400 dilution
Lanes 1-4 : HeLa cell extract cytoplasmic fraction
Lanes 5-8 : HeLa cell extract nuclear fraction
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : IRDye800™ secondary antibody at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Datasheets and documents
-
SDS download
-
Datasheet download
References (17)
ab191332 has been referenced in 17 publications.
- Pinto IF et al. Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production. ACS Sens 6:842-851 (2021). PubMed: 33724791
- Hoshino A et al. Cytosolic p53 inhibits Parkin-mediated mitophagy and promotes mitochondrial dysfunction in the mouse heart. Nat Commun 4:2308 (2013). PubMed: 23917356
- Iguchi M et al. Parkin-catalyzed ubiquitin-ester transfer is triggered by PINK1-dependent phosphorylation. J Biol Chem 288:22019-32 (2013). PubMed: 23754282
- Hussien R & Brooks GA Mitochondrial and plasma membrane lactate transporter and lactate dehydrogenase isoform expression in breast cancer cell lines. Physiol Genomics 43:255-64 (2011). PubMed: 21177384
- Matsuda N et al. PINK1 stabilized by mitochondrial depolarization recruits Parkin to damaged mitochondria and activates latent Parkin for mitophagy. J Cell Biol 189:211-21 (2010). WB ; Human . PubMed: 20404107