Product nameAnti-Lactate Dehydrogenase antibody
See all Lactate Dehydrogenase primary antibodies
DescriptionGoat polyclonal to Lactate Dehydrogenase
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Rabbit, Human
Predicted to work with: Mammals
Full length native protein (purified) corresponding to Rabbit Lactate Dehydrogenase aa 2-332. (Isolated from rabbit muscle).
AALKDQLIHNLLKEEHVPQNKITVVGVGAVGMACAISILMKDLADELALV DVMEDKLKGEMMDLQHGSLFLRTPKIVSGKDYSVTANSKLVIITAGARQQ EGESRLNLVQRNVNIFKFIIPNVVKYSPHCKLLVVSNPVDILTYVAWKIS GFPKNRVIGSGCNLDSARFRYLMGERLGVHALSCHGWILGEHGDSSVPVW SGMNVAGVSLKTLHPELGTDADKEQWKQVHKQVVDSAYEVIKLKGYTTWA IGLSVADLAESIMKNLRRVHPISTMLKGLYGIKEDVFLSVPCVLGQNGIS DVVKVTLTSEEEAHLKKSADTLWGIQKELQF
Database link: P13491
- Cytoplasmic and nuclear HeLa cell fractions.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Potassium phosphate
Concentration information loading...
Purification notesab191332 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer.
Our Abpromise guarantee covers the use of ab191332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).|
PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in diseaseDefects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.
- Information by UniProt
- Cell proliferation-inducing gene 19 protein antibody
- GSD11 antibody
- L lactate dehydrogenase B chain antibody
All lanes : Anti-Lactate Dehydrogenase antibody (ab191332) at 1/400 dilution
Lanes 1-4 : HeLa cell extract cytoplasmic fraction
Lanes 5-8 : HeLa cell extract nuclear fraction
Lysates/proteins at 30 µg per lane.
All lanes : IRDye800™ secondary antibody at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Hoshino A et al. Cytosolic p53 inhibits Parkin-mediated mitophagy and promotes mitochondrial dysfunction in the mouse heart. Nat Commun 4:2308 (2013). Read more (PubMed: 23917356) »
- Iguchi M et al. Parkin-catalyzed ubiquitin-ester transfer is triggered by PINK1-dependent phosphorylation. J Biol Chem 288:22019-32 (2013). Read more (PubMed: 23754282) »