Overview

  • Product name
    Anti-Lactate Dehydrogenase antibody (Biotin)
    See all Lactate Dehydrogenase primary antibodies
  • Description
    Goat polyclonal to Lactate Dehydrogenase (Biotin)
  • Host species
    Goat
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: ELISA, Immunomicroscopy, WB, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Human, Chinese hamster
    Predicted to work with: a wide range of other species
  • Immunogen

    Full length native Lactate Dehydrogenase (purified).

  • Positive control
    • purified enzyme from rabbit muscle(Worthington Biochemicals)
  • General notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.

Properties

Applications

Our Abpromise guarantee covers the use of ab7638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Immunomicroscopy
WB
Dot blot
  • Application notes
    Dot: Use at an assay dependent dilution.
    ELISA: 1/4000 - 1/20000.
    IM: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Please note: The non-conjugated version of this antibody (ab2101) has been used in WB in the reference Zaman K et al (protocol available on the datasheet for ab2101).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Pathway
      Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
    • Involvement in disease
      Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
    • Sequence similarities
      Belongs to the LDH/MDH superfamily. LDH family.
    • Post-translational
      modifications
      ISGylated.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cell proliferation-inducing gene 19 protein antibody
      • GSD11 antibody
      • L lactate dehydrogenase B chain antibody
      • L-lactate dehydrogenase A chain antibody
      • Lactate dehydrogenase A antibody
      • Lactate dehydrogenase B antibody
      • Lactate dehydrogenase H chain antibody
      • Lactate dehydrogenase M antibody
      • LDH A antibody
      • LDH B antibody
      • LDH H antibody
      • LDH heart subunit antibody
      • LDH M antibody
      • LDH muscle subunit antibody
      • LDH-A antibody
      • LDH-M antibody
      • LDH1 antibody
      • ldha antibody
      • LDHA_HUMAN antibody
      • LDHBD antibody
      • LDHM antibody
      • MS1111 antibody
      • PIG19 antibody
      • Proliferation inducing gene 19 antibody
      • Renal carcinoma antigen NY REN 46 antibody
      • Renal carcinoma antigen NY-REN-59 antibody
      • TRG 5 antibody
      • TRG5 antibody
      see all

    References

    This product has been referenced in:
    • Kim SH  et al. PGC-1a mediates a rapid, exercise-induced downregulation of glycogenolysis in rat skeletal muscle. J Physiol 593:635-43 (2015). WB ; Rat . Read more (PubMed: 25416622) »
    • Rudyk O  et al. Protein kinase G oxidation is a major cause of injury during sepsis. Proc Natl Acad Sci U S A 110:9909-13 (2013). Mouse . Read more (PubMed: 23716652) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ELISA
    Sample
    Chinese Hamster Cell (Ovary)
    Specification
    Ovary
    Type
    Direct

    Abcam user community

    Verified customer

    Submitted Apr 11 2012

    Answer

    I am very pleased to hear the customer would like to accept our offer and test ab7638 in Chinese hamster.

    DISCOUNT CODE: #####
    Expiration date: ######

    This code will give you 1 free primary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for Chinese hamster and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site:

    www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Any feedback that the customer can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receivingthe customers Abreview and wish them luck with their research.

    Read More

    Answer

    Thank you for your enquiry.

    I am sorry to confirm that as far as we are aware, ab7638 Lactate Dehydrogenase antibody (Biotin)] has never been tested with samples from Chinese hamster. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

    I have checked the alignment of the immunogen (from rabbit) with the Chinese hamster lactate dehydrogenase sequence. The alignment is 93%. This is high, and it is likely the antibody will detect in this species. However, I am sorry we cannot guarantee this without further testing.

    If the customerwould like to test the antibody in this untested species, please contact me again by replying to this message prior to the purchase asthey may be eligible for our testing discount program.

    Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

    To find out more about our Abreview system, please see the following link:

    https://www.abcam.com/abreviews

    I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

    Read More

    Answer

    Unfortunately, this information is not known.

    Read More

    Answer

    The unconjugated form (ab2101) was raised against rabbit LDH. This is expected to react with other species, although this has not been tested yet.

    Read More

    Answer

    ab7638 is linked to biotin. For Western blotting procedures such as this we would recommend ab2101 followed by a secondary against goat linked to HRP (such as ab7125).

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up