Product nameAnti-Lactate Dehydrogenase antibody (Biotin)
See all Lactate Dehydrogenase primary antibodies
DescriptionGoat polyclonal to Lactate Dehydrogenase (Biotin)
Tested applicationsSuitable for: ELISA, Immunomicroscopy, WB, Dot blotmore details
Species reactivityReacts with: Mouse, Rabbit, Human, Chinese hamster
Predicted to work with: a wide range of other species
Full length native Lactate Dehydrogenase (purified).
- purified enzyme from rabbit muscle(Worthington Biochemicals)
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab7638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/4000 - 1/20000.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Please note: The non-conjugated version of this antibody (ab2101) has been used in WB in the reference Zaman K et al (protocol available on the datasheet for ab2101).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in diseaseDefects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.
- Information by UniProt
- Cell proliferation-inducing gene 19 protein antibody
- GSD11 antibody
- L lactate dehydrogenase B chain antibody
This product has been referenced in:
- Kim SH et al. PGC-1a mediates a rapid, exercise-induced downregulation of glycogenolysis in rat skeletal muscle. J Physiol 593:635-43 (2015). WB ; Rat . Read more (PubMed: 25416622) »
- Rudyk O et al. Protein kinase G oxidation is a major cause of injury during sepsis. Proc Natl Acad Sci U S A 110:9909-13 (2013). Mouse . Read more (PubMed: 23716652) »