Overview

  • Product name

    Anti-Lactate Dehydrogenase antibody [EP1563Y]
    See all Lactate Dehydrogenase primary antibodies
  • Description

    Rabbit monoclonal [EP1563Y] to Lactate Dehydrogenase
  • Host species

    Rabbit
  • Specificity

    ab134187 can recognize LDH-A, B, and C.
  • Tested applications

    Suitable for: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Lactate Dehydrogenase aa 1-100.

  • Positive control

    • A549 cell lysate; Human kidney carcinoma
  • General notes

    For Mouse, this antibody is recommended for WB only.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Predicted molecular weight: 37 kDa.
IP 1/70.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • Anti-Lactate Dehydrogenase antibody [EP1563Y] (ab134187) at 1/1000 dilution + A549 cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 37 kDa

  • Immunohistochemical analysis of paraffin-embedded Human kidney carcinoma labelling Lactate Dehydrogenase with ab134187 at 1/100 dilution.

References

This product has been referenced in:

  • Infante RE & Radhakrishnan A Continuous transport of a small fraction of plasma membrane cholesterol to endoplasmic reticulum regulates total cellular cholesterol. Elife 6:N/A (2017). Read more (PubMed: 28414269) »
  • Jin X  et al. HO-1/EBP interaction alleviates cholesterol-induced hypoxia through the activation of the AKT and Nrf2/mTOR pathways and inhibition of carbohydrate metabolism in cardiomyocytes. Int J Mol Med 39:1409-1420 (2017). Read more (PubMed: 28487965) »
See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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