For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    lactate-dehydrogenase-antibody-ep1566y-ab52488.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Metabolism Energy Metabolism
Share by email
RecombinantRabMAb

Recombinant Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

  • Datasheet
  • SDS
Reviews (7)Q&A (1)References (38)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
  • Flow Cytometry - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
  • Immunoprecipitation - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
  • Western blot - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
  • Western blot - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
  • Flow Cytometry - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
  • Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1566Y] to Lactate Dehydrogenase
  • Suitable for: ICC/IF, WB, IP, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Assay
Product image
Extracellular Oxygen Consumption Reagent (ab197242)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant rat Lactate Dehydrogenase protein (Active) (ab222427)

View more associated products

Overview

  • Product name

    Anti-Lactate Dehydrogenase antibody [EP1566Y]
    See all Lactate Dehydrogenase primary antibodies
  • Description

    Rabbit monoclonal [EP1566Y] to Lactate Dehydrogenase
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with Lactate dehydrogenase; LDHA (79%), LDHB (100%) and LDHC (86%).

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Lactate Dehydrogenase aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P07195

  • Positive control

    • WB: HeLa, HEK293, human heart tissue; rat and mouse kidney tissue lysate. ICC/IF: HeLa cells. Flow cyt: Raw264.7 cells, HeLa. IP: HeLa cells. IHC-P: Mouse liver tissue, human breast carcinoma tissue, human liver carcinoma.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1566Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Lactic Dehydrogenase
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab202652)
    • HRP Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab202653)
    • Alexa Fluor® 594 Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab214857)
    • Anti-Lactate Dehydrogenase antibody [EP1566Y] - BSA and Azide free (ab219591)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant rat Lactate Dehydrogenase protein (Active) (ab222427)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Mouse
Human
ICC/IF
Human
IHC-P
Mouse
Human
IP
Human
WB
Mouse
Rat
Human
Application Abreviews Notes
ICC/IF (1)
1/50 - 1/100.
WB (3)
1/5000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IP
1/30 - 1/100.
Flow Cyt
1/50 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P (2)
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Notes
ICC/IF
1/50 - 1/100.
WB
1/5000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
IP
1/30 - 1/100.
Flow Cyt
1/50 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Pathway

    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • Involvement in disease

    Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
  • Sequence similarities

    Belongs to the LDH/MDH superfamily. LDH family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P00338 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3939 Human
    • Entrez Gene: 3945 Human
    • Entrez Gene: 16828 Mouse
    • Entrez Gene: 16832 Mouse
    • Entrez Gene: 24533 Rat
    • Entrez Gene: 24534 Rat
    • Omim: 150000 Human
    • Omim: 150100 Human
    • SwissProt: P00338 Human
    • SwissProt: P07195 Human
    • SwissProt: P07864 Human
    • SwissProt: P06151 Mouse
    • SwissProt: P16125 Mouse
    • SwissProt: P04642 Rat
    • SwissProt: P42123 Rat
    • Unigene: 2795 Human
    • Unigene: 29324 Mouse
    • Unigene: 107896 Rat
    see all
  • Alternative names

    • Cell proliferation-inducing gene 19 protein antibody
    • GSD11 antibody
    • L lactate dehydrogenase B chain antibody
    • L-lactate dehydrogenase A chain antibody
    • Lactate dehydrogenase A antibody
    • Lactate dehydrogenase B antibody
    • Lactate dehydrogenase c variant 1 antibody
    • Lactate dehydrogenase c variant 3 antibody
    • Lactate dehydrogenase c variant 4 antibody
    • Lactate dehydrogenase C4 antibody
    • Lactate dehydrogenase H chain antibody
    • Lactate dehydrogenase M antibody
    • LDH A antibody
    • LDH B antibody
    • LDH H antibody
    • LDH heart subunit antibody
    • LDH M antibody
    • LDH muscle subunit antibody
    • LDH-A antibody
    • LDH-M antibody
    • LDH1 antibody
    • ldha antibody
    • LDHA_HUMAN antibody
    • LDHBD antibody
    • LDHM antibody
    • MS1111 antibody
    • PIG19 antibody
    • Proliferation inducing gene 19 antibody
    • Proliferation-inducing gene 19 antibody
    • Renal carcinoma antigen NY REN 46 antibody
    • Renal carcinoma antigen NY-REN-59 antibody
    • TRG 5 antibody
    • TRG5 antibody
    see all

Images

  • Western blot - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    Western blot - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    All lanes : Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488) at 1/5000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK293 (human embryonic kidney) whole cell lysate
    Lane 3 : Human heart tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 37 kDa
    Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

    ab52488 staining Lactate Dehydrogenase in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab52488 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

  • Flow Cytometry - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    Flow Cytometry - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

    Flow Cytometry analysis of Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate labelling Lactate Dehydrogenase with purified ab52488 at 1/190 (red). Cells were fixed with 4% paraformaldehyde. Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Immunoprecipitation - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    Immunoprecipitation - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

    ab52488 immunoprecipitating Lactate Dehydrogenase. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/30 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.

    Lane 1: HeLa (human cervix adenocarcinoma) whole cell lysate (10ug)
    Lane 2: HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab52488 in HeLa (human cervix adenocarcinoma) whole cell lysate

  • Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

    ICC/IF image of unpurified ab52488 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52488, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

    ab52488 staining Lactate Dehydrogenase in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

    ab52488 staining Lactate Dehydrogenase in human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. ab97051 was used as the secondary antibody.

    Negative control 1: PBS in place of primary antibody.

  • Western blot - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    Western blot - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    All lanes : Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488) at 1/20000 dilution (purified)

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Rat kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer 5% NFDM/TBST
  • Western blot - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    Western blot - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488) at 1/100000 dilution (unpurified) + Hela cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

    Immunohistochemical analysis of paraffin-embedded human liver carcinoma using unpurified ab52488 at a 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

    ab52488 staining Lactate Dehydrogenase in mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/2000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Flow Cytometry - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    Flow Cytometry - Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

    Overlay histogram showing HeLa cells stained with unpurified ab52488 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52488, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)
    Anti-Lactate Dehydrogenase antibody [EP1566Y] (ab52488)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (38)

Publishing research using ab52488? Please let us know so that we can cite the reference in this datasheet.

ab52488 has been referenced in 38 publications.

  • Famulari ES  et al. Human liver stem cells express UGT1A1 and improve phenotype of immunocompromised Crigler Najjar syndrome type I mice. Sci Rep 10:887 (2020). PubMed: 31965023
  • Pires Da Silva J  et al. SIRT1 Protects the Heart from ER Stress-Induced Injury by Promoting eEF2K/eEF2-Dependent Autophagy. Cells 9:N/A (2020). PubMed: 32059483
  • Eriksson I  et al. Restoration of lysosomal function after damage is accompanied by recycling of lysosomal membrane proteins. Cell Death Dis 11:370 (2020). PubMed: 32409651
  • Cardoso HJ  et al. Tyrosine kinase inhibitor imatinib modulates the viability and apoptosis of castrate-resistant prostate cancer cells dependently on the glycolytic environment. Life Sci 218:274-283 (2019). PubMed: 30605651
  • Le Page LM  et al. Assessing the effect of hypoxia on cardiac metabolism using hyperpolarized 13 C magnetic resonance spectroscopy. NMR Biomed 32:e4099 (2019). PubMed: 31090979
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-8 of 8 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Lactate Dehydrogenase antibody [EP1566Y]

Excellent
Abreviews
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Lung adenocarcinoma)
Permeabilization
No
Specification
Lung adenocarcinoma
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 8% · Temperature: 27°C
Fixative
Paraformaldehyde
Read More

Dr. Shubhangi Agarwal

Verified customer

Submitted Jan 31 2018

Western blot abreview for Anti-Lactate Dehydrogenase antibody [EP1566Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12 cells)
Gel Running Conditions
Reduced Denaturing (9%)
Loading amount
30 µg
Treatment
2mM Metformin for 24hr
Specification
C2C12 cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jan 13 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lactate Dehydrogenase antibody [EP1566Y]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Apr 18 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lactate Dehydrogenase antibody [EP1566Y]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Apr 18 2016

Western blot abreview for Anti-Lactate Dehydrogenase antibody [EP1566Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Spinal Cord)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Spinal Cord
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jun 26 2015

Western blot abreview for Anti-Lactate Dehydrogenase antibody [EP1566Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Brain Tissue)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Brain Tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jun 22 2015

Question

- searching reactives for IHC-P of LDH 5 - including revelation kit, blocking reagents and antigen retrieval solution

Read More

Abcam community

Verified customer

Asked on Jul 12 2012

Answer

Merci pour votre appel de hier. Je suis désolée du retard de ma réponse.

Voici donc la liste des produits que nous avions discuté ainsi que les liens pour les protocoles que nous avons en ligne.

Les anticorps qui ont été testé contre la protéine humaine ainsi que sur coupes paraffine:
contre la LDH total, c'est le ab52488 et contre la LDH isoenzyme V c'est le ab101562.
https://www.abcam.com/Lactate-Dehydrogenase-antibody-EP1566Y-ab52488.html (or use the following: https://www.abcam.com/Lactate-Dehydrogenase-antibody-EP1566Y-ab52488.html).
https://www.abcam.com/LDHA-antibody-EPR1564-ab101562.html (or use the following: https://www.abcam.com/LDHA-antibody-EPR1564-ab101562.html).

Le kit de révélation pour détecter l'anticorps primaire est le kit ab80436. Comme vous utilisez ce kit, vous n'avez pas besoin de bloquer la biotine endogène ni d'un autre agent bloquant les protéines, car il contient déjà la solution de blocage ("protein block").
https://www.abcam.com/EXPOSE-Mouse-and-Rabbit-Specific-HRPDAB-Detection-IHC-Kit-ab80436.html (or use the following: https://www.abcam.com/EXPOSE-Mouse-and-Rabbit-Specific-HRPDAB-Detection-IHC-Kit-ab80436.html).

Les solutions de démasquages sont le PH 9 ab93684 pour un démasquage à pH 9 et le ab93678 pour un démasquage à pH 6.
https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Tris-EDTA-Buffer-pH-90-ab93684.html (or use the following: https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Tris-EDTA-Buffer-pH-90-ab93684.html).
https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Citrate-Buffer-pH-60-ab93678.html (or use the following: https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Citrate-Buffer-pH-60-ab93678.html).

La solution de blocage est le ab64218 pour bloquer l'activité endogène de peroxydase. Cet étape de blocage est très important si vous travaillez avec des tissues qui contiennent de l'activité endogène de peroxydase, comme par exemple le poumon. Veuillez ne pas hésiter à nous rappeler pour discuter ceci en plus de détails.
https://www.abcam.com/Hydrogen-Peroxide-Blocking-Reagent-ab64218.html (or use the following: https://www.abcam.com/Hydrogen-Peroxide-Blocking-Reagent-ab64218.html).

Voici les liens pour les protocoles d'immunohistochimie. Ils contiennent beaucoup des informations utiles.

https://www.abcam.com/index.html?pageconfig=resource&rid=11757
le même protocole en pdf: https://www.abcam.com/ps/pdf/protocols/ihc_p.pdf
https://www.abcam.com/index.html?pageconfig=resource&rid=11384
https://www.abcam.com/index.html?pageconfig=popular_protocols

Voici aussi ici des liens plus spécifiques, comme pour le démasquage et pour les kits de détection.
https://www.abcam.com/index.html?pageconfig=searchresults&pt=6&search=antigen retrieval
https://www.abcam.com/index.html?pageconfig=resource&rid=12865&source=pagetrap&viapagetrap=expose

J'espère que ces informations vous sont utiles. N'hésitez pas à nous rappeler pour plus d'information.

Read More

Abcam Scientific Support

Answered on Jul 13 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-Lactate Dehydrogenase antibody [EP1566Y]

Excellent
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (pancreas)
Specification
pancreas
Fixative
acetone:methonal (1:1)
Permeabilization
Yes - triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Oct 26 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2021 Abcam plc. All rights reserved.