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Mouse monoclonal [10E6AA9] to Lactate Dehydrogenase B / LDH-B
Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.
Flow Cyt more details
Tissue, cells or virus corresponding to Lactate Dehydrogenase B/ LDH-B.
HeLa cells and cell lysate.
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact
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Shipped at 4°C. Store at +4°C. Do Not Freeze.
Preservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline
Concentration information loading...
>95% by SDS-PAGE
ab112996 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE.
Light chain type
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml.
Use at an assay dependent concentration.
Use a concentration of 2 µg/ml.
Use a concentration of 1 - 2 µg/ml.
Triton permeabilization required.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in disease
Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease.
Belongs to the LDH/MDH superfamily. LDH family.
Information by UniProt
Epididymis secretory protein Li 281 antibody
HEL S 281 antibody
L lactate dehydrogenase B chain antibody
Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase B / LDH-B antibody [10E6AA9] (ab112996)
ab112996 at 2ug/ml staining Lactate Dehydrogenase B / LDH-B in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). The target protein locates mainly to the cytoplasm.
Flow Cytometry - Anti-Lactate Dehydrogenase B / LDH-B antibody [10E6AA9] (ab112996)
ab112996 at 1ug/ml staining Lactate Dehydrogenase B / LDH-B in Triton X-100 permeabilized HeLa cells by flow cytometry (blue). Isotype control antibody (red).
Immunoprecipitation - Anti-Lactate Dehydrogenase B / LDH-B antibody [10E6AA9] (ab112996)
Isolation of Lactate Dehydrogenase B / LDH-B using ab112996 by immunoprecipitation.
The identity of the protein was confirmed by mass spectrometry. The gel was stained with colloidal Coomassie blue G.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"