Anti-Lactate Dehydrogenase B antibody [EP1565Y] (HRP) (ab208366)

Overview

  • Product name
    Anti-Lactate Dehydrogenase B antibody [EP1565Y] (HRP)
    See all Lactate Dehydrogenase B primary antibodies
  • Description
    Rabbit monoclonal [EP1565Y] to Lactate Dehydrogenase B (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Lactate Dehydrogenase B aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P07195

  • Positive control
    • WB: HeLa whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab208366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).

Target

  • Pathway
    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • Involvement in disease
    Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease.
  • Sequence similarities
    Belongs to the LDH/MDH superfamily. LDH family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis secretory protein Li 281 antibody
    • HEL S 281 antibody
    • L lactate dehydrogenase B chain antibody
    • L-lactate dehydrogenase B chain antibody
    • Lactate dehydrogenase H chain antibody
    • LDH B antibody
    • LDH H antibody
    • LDH heart subunit antibody
    • LDH-B antibody
    • LDH-H antibody
    • LDHB antibody
    • LDHB_HUMAN antibody
    • LDHBD antibody
    • LDHH antibody
    • Renal carcinoma antigen NY REN 46 antibody
    • Renal carcinoma antigen NY-REN-46 antibody
    • TRG-5 antibody
    • TRG5 antibody
    see all

Images

  • Anti-Lactate Dehydrogenase B antibody [EP1565Y] (HRP) (ab208366) at 1/5000 dilution + HeLa whole cell lysate (ab150035) at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 35 kDa (why is the actual band size different from the predicted?)


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208366 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab208366 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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