Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)
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Overview
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Product nameAnti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9]
See all Lactate Dehydrogenase B/LDH-B primary antibodies -
DescriptionMouse monoclonal [10E6AA9] to Lactate Dehydrogenase B/LDH-B
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Host speciesMouse
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SpecificityShotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.
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Tested applicationsSuitable for: ICC/IF, IP, In-Cell ELISA, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Tissue, cells or virus corresponding to Lactate Dehydrogenase B/LDH-B.
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Positive control
- HeLa cells and cell lysate.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Previously labelled as Lactate Dehydrogenase B.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline -
Concentration information loading... -
Purity>95% by SDS-PAGE
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Purification notesab112996 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE.
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ClonalityMonoclonal
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Clone number10E6AA9
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab112996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 2 µg/ml. | |
| IP | Use at an assay dependent concentration. | |
| In-Cell ELISA | Use a concentration of 2 µg/ml. | |
| Flow Cyt | Use a concentration of 1 - 2 µg/ml. Triton permeabilization required.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
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Involvement in diseaseNote=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease.
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Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.
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Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 3945 Human
- Omim: 150100 Human
- SwissProt: P07195 Human
- Unigene: 446149 Human
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Alternative names
- Epididymis secretory protein Li 281 antibody
- HEL S 281 antibody
- L lactate dehydrogenase B chain antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)](https://www.abcam.com/ps/products/112/ab112996/Images/Lactate-Dehydrogenase-B-Primary-antibodies-ab112996-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)ab112996 at 2ug/ml staining Lactate Dehydrogenase B/LDH-B in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). The target protein locates mainly to the cytoplasm.
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![Flow Cytometry - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)](https://www.abcam.com/ps/products/112/ab112996/Images/Lactate-Dehydrogenase-B-Primary-antibodies-ab112996-2.jpg)
Flow Cytometry - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)ab112996 at 1ug/ml staining Lactate Dehydrogenase B/LDH-B in Triton X-100 permeabilized HeLa cells by flow cytometry (blue). Isotype control antibody (red).
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![Immunoprecipitation - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)](https://www.abcam.com/ps/products/112/ab112996/Images/Lactate-Dehydrogenase-B-Primary-antibodies-ab112996-3.jpg)
Immunoprecipitation - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)Isolation of Lactate Dehydrogenase B/LDH-B using ab112996 by immunoprecipitation.
The identity of the protein was confirmed by mass spectrometry. The gel was stained with colloidal Coomassie blue G.
Protocols
Datasheets and documents
References
ab112996 has not yet been referenced specifically in any publications.
