Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)
![Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)](https://www.abcam.com/ps/products/112/ab112996/Images/ab112996-125716-anti-lactate-dehydrogenase-b-ldh-b-antibody-10e6aa9-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)")
Key features and details
- Mouse monoclonal [10E6AA9] to Lactate Dehydrogenase B/LDH-B
- Suitable for: ICC/IF, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9]
See all Lactate Dehydrogenase B/LDH-B primary antibodies -
Description
Mouse monoclonal [10E6AA9] to Lactate Dehydrogenase B/LDH-B -
Host species
Mouse -
Specificity
Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques. -
Tested applications
Suitable for: ICC/IF, IP, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- HeLa cells and cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
ab112996 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%). -
Clonality
Monoclonal -
Clone number
10E6AA9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 2 µg/ml.
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| IP |
Use at an assay dependent concentration.
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| Flow Cyt |
Use a concentration of 1 - 2 µg/ml.
Triton permeabilization required. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
Use a concentration of 2 µg/ml. |
|
IP
Use at an assay dependent concentration. |
|
Flow Cyt
Use a concentration of 1 - 2 µg/ml. Triton permeabilization required. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
Involvement in disease
Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease. -
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3945 Human
- Omim: 150100 Human
- SwissProt: P07195 Human
- Unigene: 446149 Human
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Alternative names
- Epididymis secretory protein Li 281 antibody
- HEL S 281 antibody
- L lactate dehydrogenase B chain antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)
ab112996 at 2ug/ml staining Lactate Dehydrogenase B/LDH-B in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). The target protein locates mainly to the cytoplasm.
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![Flow Cytometry - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)](https://www.abcam.com/ps/products/112/ab112996/Images/ab112996-125715-anti-lactate-dehydrogenase-b-ldh-b-antibody-10e6aa9-flow-cytometry.jpg)
Flow Cytometry - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)ab112996 at 1ug/ml staining Lactate Dehydrogenase B/LDH-B in Triton X-100 permeabilized HeLa cells by flow cytometry (blue). Isotype control antibody (red).
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![Immunoprecipitation - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)](https://www.abcam.com/ps/products/112/ab112996/Images/ab112996-125714-anti-lactate-dehydrogenase-b-ldh-b-antibody-10e6aa9-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)Isolation of Lactate Dehydrogenase B/LDH-B using ab112996 by immunoprecipitation.
The identity of the protein was confirmed by mass spectrometry. The gel was stained with colloidal Coomassie blue G.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab112996 has been referenced in 1 publication.
- Ran F et al. miR-1224-3p Promotes Breast Cancer Cell Proliferation and Migration through PGM5-Mediated Aerobic Glycolysis. J Oncol 2021:5529770 (2021). PubMed: 33986801
