Question (30509) | Anti-Lactate Dehydrogenase B/LDH-B antibody [60H11] (ab85319)

Go to datasheet (ab85319)

Question

Attached is the blotting I got today following the protocol provided online with a recent antibody of ab85319. Lane 1 is the protein marker, lane2-5 are different rat samples. I wonder whether you can offer me some suggestions to improve the blotting. Sincerely,  

Answer

Thank you for contacting us with your question. I could not find the image attached to your email- could you please resend it? I also have a few questions so that I can better help you: 1) Do you have your original order number or PO number? 2) How much protein do you load onto the blot? 3) What kind of rat samples have you tried? 4) What blocking solution (milk or BSA) was used? 5) What dilution of antibody was used? I look forward to hearing from you and resolving this promptly. Please let me know if you have any questions or if there is anything else that we can do for you.

Sign up