Question (30666) | Anti-Lactate Dehydrogenase B/LDH-B antibody [60H11] (ab85319)

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Question

Dear Abcam team, I am sorry that the image was not attached. I do not have the original PO number. It was ordered around two months ago or one month ago. I loaded 30 ug total protein per well. The samples were from immortalized rat renal proximal tubule cells. The blocking buffer was casein/PBS (BioRad), the antibody was diluted in 4%BSA (1:500 dilution). Thank you very much for your help

Answer

Thank you for your reply and for sending the image again with your protocol information. There does seem to be a lot of background on the blot, but this can be reduced by further diluting the antibody (we recommend 1:5000-1:10000). Once the background is reduced, the specific band should be more clear. If the problem persists however, I would be happy to send a different LDHB antibody for you to try. I hope this information will be useful, but please let me know if you have any questions and I'll be happy to help. Please keep me updated about any further results with this antibody, and I look forward to hearing from you.

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