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I was wondering if you could let me know the specificity of 2 antibodies. I am looking at antibodies against LDHa and LDHb. Specifically products ab4710 and ab85319. I would like to know the cross-reactivity between the two isoforms, meaning does the LDHa antibody detect LDHb? And vice-versa.
Asked on Sep 17 2012
The antibody ab85319, anti-LDHb, reacts with western blots of recombinant LDHb but not blots containing recombinant LDHa. Please let us know if you have any questions.
As my colleague mentioned in a previous message, ab4710 is not an antibody, and I am guessing you meant ab47010. To our knowledge, there has been no testing against purified LDHb, but based on the immunogen sequence, it should not be capable of detecting LDHb.
Answered on Sep 17 2012