Western blot abreview for Anti-Lactate Dehydrogenase B/LDH-B antibody [60H11]

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
3e+006 cells
Specification
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% milk in TBS-T
Dilution
1/500

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: A16078 (Invitrogen)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 40 kDa Non-specific: 60 & 70 kDa
Positive control
Total extracts

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Submitted Nov 10 2017

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