Key features and details
- Goat polyclonal to Lactate Dehydrogenase B/LDH-B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Lactate Dehydrogenase B/LDH-B antibody
See all Lactate Dehydrogenase B/LDH-B primary antibodies
DescriptionGoat polyclonal to Lactate Dehydrogenase B/LDH-B
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Guinea pig, Chimpanzee, Monkey, Gorilla, Orangutan
- Human heart and kidney lysates; Human kidney tissue.
Previously labelled as Lactate Dehydrogenase B.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab195964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).|
|IHC-P||Use a concentration of 5 µg/ml.|
PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in diseaseNote=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease.
Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.
- Information by UniProt
- Epididymis secretory protein Li 281 antibody
- HEL S 281 antibody
- L lactate dehydrogenase B chain antibody
All lanes : Anti-Lactate Dehydrogenase B/LDH-B antibody (ab195964) at 0.3 µg/ml
Lane 1 : Human heart lysate (in RIPA buffer)
Lane 2 : Human kidney lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
Immunohistochemical anaysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling Lactate Dehydrogenase B/LDH-B with ab195964 at 5 µg/mL.
ab195964 has not yet been referenced specifically in any publications.