Overview

  • Product name
    Anti-Lactate Dehydrogenase B/LDH-B antibody
    See all Lactate Dehydrogenase B/LDH-B primary antibodies
  • Description
    Goat polyclonal to Lactate Dehydrogenase B/LDH-B
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Guinea pig, Chimpanzee, Monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Lactate Dehydrogenase B/LDH-B aa 15-25 (internal sequence) (Cysteine residue). (Near N-terminus; NP_002291.1).
    Sequence:

    EEATVPNNKIT


    Database link: P07195

  • Positive control
    • Human heart and kidney lysates; Human kidney tissue.
  • General notes

    Previously labelled as Lactate Dehydrogenase B.

Properties

Applications

Our Abpromise guarantee covers the use of ab195964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Pathway
    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • Involvement in disease
    Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease.
  • Sequence similarities
    Belongs to the LDH/MDH superfamily. LDH family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Epididymis secretory protein Li 281 antibody
    • HEL S 281 antibody
    • L lactate dehydrogenase B chain antibody
    • L-lactate dehydrogenase B chain antibody
    • Lactate Dehydrogenase B antibody
    • Lactate dehydrogenase H chain antibody
    • LDH B antibody
    • LDH H antibody
    • LDH heart subunit antibody
    • LDH-B antibody
    • LDH-H antibody
    • LDHB antibody
    • LDHB_HUMAN antibody
    • LDHBD antibody
    • LDHH antibody
    • Renal carcinoma antigen NY REN 46 antibody
    • Renal carcinoma antigen NY-REN-46 antibody
    • TRG-5 antibody
    • TRG5 antibody
    see all

Images

  • All lanes : Anti-Lactate Dehydrogenase B/LDH-B antibody (ab195964) at 0.3 µg/ml

    Lane 1 : Human heart lysate (in RIPA buffer)
    Lane 2 : Human kidney lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 37 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

  • Immunohistochemical anaysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling  Lactate Dehydrogenase B/LDH-B  with ab195964 at 5 µg/mL.

References

ab195964 has not yet been referenced specifically in any publications.

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