Recombinant Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] - BSA and Azide free (ab244228)
![Western blot - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)](https://www.abcam.com/ps/products/244/ab244228/Images/ab244228-445541-anti-lactate-dehydrogenase-antibody-ep1565y-bsa-and-azide-free-western-blot-human-lysate.jpg "Western blot - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1565Y] to Lactate Dehydrogenase B/LDH-B - BSA and Azide free
- Suitable for: IHC-P, WB, IP, ICC, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] - BSA and Azide free
See all Lactate Dehydrogenase B/LDH-B primary antibodies -
Description
Rabbit monoclonal [EP1565Y] to Lactate Dehydrogenase B/LDH-B - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IP, ICC, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab244228 is the carrier-free version of ab53292.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1565Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab244228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
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| IP |
Use at an assay dependent concentration.
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| ICC |
Use at an assay dependent concentration.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
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IP
Use at an assay dependent concentration. |
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ICC
Use at an assay dependent concentration. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
Involvement in disease
Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease. -
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3945 Human
- Omim: 150100 Human
- SwissProt: P07195 Human
- Unigene: 446149 Human
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Alternative names
- Epididymis secretory protein Li 281 antibody
- HEL S 281 antibody
- L lactate dehydrogenase B chain antibody
see all
Images
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Western blot - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)All lanes : Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292) at 1/5000 dilution
Lane 1 : Wild-type Hap1 cell lysate
Lane 2 : LDHB knockout Hap1 cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : LDHB knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 37 kDaThis data was developed using ab53292, the same antibody clone in a different buffer formulation.
Lanes 1 - 4: Merged signal (red and green). Green - ab53292 observed at 37 kDa. Red - loading control, ab130007 observed at 125 kDa.
ab53292 was shown to react with Lactate Dehydrogenase in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255403 (knockout cell lysate ab263761) was used. Wild-type and Lactate Dehydrogenase knockout samples were subjected to SDS-PAGE. ab53292 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)](https://www.abcam.com/ps/products/244/ab244228/Images/ab244228-410559-anti-lactate-dehydrogenase-antibody-ep1565y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)All lanes : Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292) at 1/50000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : LDHB (Lactate Dehydrogenase B) knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 37 kDaThis data was developed using ab53292, the same antibody clone in a different buffer formulation.
Lanes 1 - 3: Merged signal (red and green). Green - ab53292 observed at 37 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab53292 was shown to specifically react with Lactate Dehydrogenase B in wild-type HAP1 cells as signal was lost in LDHB (Lactate Dehydrogenase B) knockout cells. Wild-type and LDHB (Lactate Dehydrogenase B) knockout samples were subjected to SDS-PAGE. ab53292 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)](https://www.abcam.com/ps/products/244/ab244228/Images/ab244228-4-anti-lactate-dehydrogenase-antibody-ep1565y-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)This data was developed using ab53292, the same antibody clone in a different buffer formulation.ICC/IF image of ab53292 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53292, neat) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)](https://www.abcam.com/ps/products/244/ab244228/Images/ab244228-3-anti-lactate-dehydrogenase-antibody-ep1565y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.png)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228) -
![Western blot - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)](https://www.abcam.com/ps/products/244/ab244228/Images/ab244228-402286-anti-lactate-dehydrogenase-antibody-ep1565y-bsa-and-azide-free-western-blot.png)
Western blot - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228) -
![Flow Cytometry - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)](https://www.abcam.com/ps/products/244/ab244228/Images/ab244228-1-anti-lactate-dehydrogenase-antibody-ep1565y-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)This data was developed using ab53292, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab53292 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53292, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)](https://www.abcam.com/ps/products/244/ab244228/Images/ab244228-6-benefits-of-recombinant-antibodies.png)
Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab244228 has not yet been referenced specifically in any publications.
