Overview

  • Product name
    Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric)
    See all Lactate Dehydrogenase kits
  • Detection method
    Fluorescent
  • Sample type
    Urine, Serum, Plasma, Other biological fluids, Adherent cells, Suspension cells, Tissue Extracts, Cell culture media
  • Assay type
    Enzyme activity
  • Sensitivity
    1 uU/ml
  • Assay time
    0h 20m
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000) provides a quick and easy method for monitoring Lactate Dehydrogenase (LDH) activity in a wide variety of samples. In this assay, LDH converts lactate into pyruvate and NADH, which reacts with the specific fluorescent probe to generate an intense fluorescent product (Ex/Em = 535/587 nm).


    This kit is simple, highly sensitive and high-throughput adaptable and can detect LDH activity as low as 1 µU/mL.


    LDH assay protocol summary:
    - add samples and standards to wells
    - add reaction mix
    - analyze with microplate reader every 2-3 min for 10-30 min

  • Notes

    Lactate dehydrogenase (LDH, L-Lactate NAD oxidoreductase, EC 1.1.1.27) is an ubiquitous enzymes among vertebrate organisms which catalyzes the reversible conversion of pyruvate to lactate, with concomitant conversion of NADH and NAD+. LDH is cytoplasmic in its cellular location and in any one tissue is composed of one or two of five possible isoenzymes. During tissue damage, LDH is released into the bloodstream; therefore it serves as a marker for various diseases and common injuries.

  • Platform
    Microplate reader

Properties

Images

  • Typical NADH Standard Curve obtained following assay protocol.

  • Kinetic measurement of Lactate Dehydrogenase activity in a range of biological samples.

  • Relative LDH Activity was calculated in lysates prepared from rat liver (0.037 µg protein), Jurkat cells (0.053 µg protein), and Human serum (0.2 µg protein).

Protocols

References

ab197000 has not yet been referenced specifically in any publications.

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