Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Adherent cells, Cell culture media, Other biological fluids, Plasma, Serum, Suspension cells, Tissue Extracts, Urine
- Sensitivity: 1 uU/ml
Product nameLactate Dehydrogenase (LDH) Assay Kit (Fluorometric)
See all Lactate Dehydrogenase kits
Sample typeUrine, Serum, Plasma, Other biological fluids, Adherent cells, Suspension cells, Tissue Extracts, Cell culture media
Assay typeEnzyme activity
Assay time0h 20m
Species reactivityReacts with: Mammals, Other species
Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000) provides a quick and easy method for monitoring Lactate Dehydrogenase (LDH) activity in a wide variety of samples. In this assay, LDH converts lactate into pyruvate and NADH, which reacts with the specific fluorescent probe to generate an intense fluorescent product (Ex/Em = 535/587 nm).
This kit is simple, highly sensitive and high-throughput adaptable and can detect LDH activity as low as 1 µU/mL.
LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader every 2-3 min for 10-30 min
Lactate dehydrogenase (LDH, L-Lactate NAD oxidoreductase, EC 22.214.171.124) is an ubiquitous enzymes among vertebrate organisms which catalyzes the reversible conversion of pyruvate to lactate, with concomitant conversion of NADH and NAD+. LDH is cytoplasmic in its cellular location and in any one tissue is composed of one or two of five possible isoenzymes. During tissue damage, LDH is released into the bloodstream; therefore it serves as a marker for various diseases and common injuries.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 500 tests LDH Assay Buffer 1 x 110ml LDH Substrate Mix 1 vial NADH Standard Yellow 1 vial PicoProbe Blue 1 x 1.4ml Positive Control 1 vial
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
PathwayFermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Involvement in diseaseDefects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
Sequence similaritiesBelongs to the LDH/MDH superfamily. LDH family.
- Information by UniProt
- Cell proliferation-inducing gene 19 protein
- L lactate dehydrogenase B chain
- SwissProt: Q7SI97 Other species
Typical NADH Standard Curve obtained following assay protocol.
Kinetic measurement of Lactate Dehydrogenase activity in a range of biological samples.
Relative LDH Activity was calculated in lysates prepared from rat liver (0.037 µg protein), Jurkat cells (0.053 µg protein), and Human serum (0.2 µg protein).
ab197000 has been referenced in 3 publications.
- Madungwe NB et al. Inner mitochondrial membrane protein MPV17 mutant mice display increased myocardial injury after ischemia/reperfusion. Am J Transl Res 12:3412-3428 (2020). Mouse . PubMed: 32774709
- Dewhurst RM et al. Development of Natural-Based Bone Cement for a Controlled Doxorubicin-Drug Release. Front Bioeng Biotechnol 8:754 (2020). PubMed: 32733869
- SevIm Ç et al. In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line. Turk J Pharm Sci 17:506-510 (2020). PubMed: 33177931