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    lactate-dehydrogenase-ldh-assay-kit-fluorometric-ab197000.pdf

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Signal Transduction Metabolism Energy Metabolism
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Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)

  • Datasheet
  • SDS
  • Protocol Booklet
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Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)
  • Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)
  • Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Assay time: 20 min
  • Sample type: Adherent cells, Cell culture media, Other biological fluids, Plasma, Serum, Suspension cells, Tissue Extracts, Urine
  • Sensitivity: 1 uU/ml

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Overview

  • Product name

    Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric)
    See all Lactate Dehydrogenase kits
  • Detection method

    Fluorescent
  • Sample type

    Urine, Serum, Plasma, Other biological fluids, Adherent cells, Suspension cells, Tissue Extracts, Cell culture media
  • Assay type

    Enzyme activity
  • Sensitivity

    1 uU/ml
  • Assay time

    0h 20m
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000) provides a quick and easy method for monitoring Lactate Dehydrogenase (LDH) activity in a wide variety of samples. In this assay, LDH converts lactate into pyruvate and NADH, which reacts with the specific fluorescent probe to generate an intense fluorescent product (Ex/Em = 535/587 nm).


    This kit is simple, highly sensitive and high-throughput adaptable and can detect LDH activity as low as 1 µU/mL.


    LDH assay protocol summary:
    - add samples and standards to wells
    - add reaction mix
    - analyze with microplate reader every 2-3 min for 10-30 min

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K730 PicoProbe™ Lactate Dehydrogenase Activity Assay Kit. K730-500 is the same size as the 500 test size of ab197000.

    Lactate dehydrogenase (LDH, L-Lactate NAD oxidoreductase, EC 1.1.1.27) is an ubiquitous enzymes among vertebrate organisms which catalyzes the reversible conversion of pyruvate to lactate, with concomitant conversion of NADH and NAD+. LDH is cytoplasmic in its cellular location and in any one tissue is composed of one or two of five possible isoenzymes. During tissue damage, LDH is released into the bloodstream; therefore it serves as a marker for various diseases and common injuries.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 500 tests
    LDH Assay Buffer 1 x 110ml
    LDH Substrate Mix 1 vial
    NADH Standard Yellow 1 vial
    PicoProbe Blue 1 x 1.4ml
    Positive Control 1 vial
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Tumor biomarkers
    • Enzymes
    • Lactic Dehydrogenase
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Sugar Assays
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Kits/ Lysates/ Other
    • Kits
    • Cell Damage Kits
    • Cell viability, plasma membrane damage
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Kits/ Lysates/ Other
    • Kits
    • Cell Damage Kits
    • Cell Damage
  • Pathway

    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
  • Involvement in disease

    Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.
  • Sequence similarities

    Belongs to the LDH/MDH superfamily. LDH family.
  • Post-translational
    modifications

    ISGylated.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P00338 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Cell proliferation-inducing gene 19 protein
    • GSD11
    • L lactate dehydrogenase B chain
    • L-lactate dehydrogenase A chain
    • Lactate dehydrogenase A
    • Lactate dehydrogenase B
    • Lactate dehydrogenase c variant 1
    • Lactate dehydrogenase c variant 3
    • Lactate dehydrogenase c variant 4
    • Lactate dehydrogenase C4
    • Lactate dehydrogenase H chain
    • Lactate dehydrogenase M
    • LDH A
    • LDH B
    • LDH H
    • LDH heart subunit
    • LDH M
    • LDH muscle subunit
    • LDH-A
    • LDH-M
    • LDH1
    • ldha
    • LDHA_HUMAN
    • LDHBD
    • LDHM
    • MS1111
    • PIG19
    • Proliferation inducing gene 19
    • Proliferation-inducing gene 19
    • Renal carcinoma antigen NY REN 46
    • Renal carcinoma antigen NY-REN-59
    • TRG 5
    • TRG5
    see all
  • Database links

    • SwissProt: Q7SI97 Other species

    Associated products

    • Assay kits

      • LDH Assay Kit / Lactate Dehydrogenase Assay Kit (Colorimetric) (ab102526)
      • LDH-Cytotoxicity Assay Kit (Fluorometric) (ab197004)
      • LDH Assay Kit (Cytotoxicity) (ab65391)
      • LDH Assay Kit (Cytotoxicity) (ab65393)

    Images

    • Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)
      Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)

      Typical NADH Standard Curve obtained following assay protocol.

    • Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)
      Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)

      Kinetic measurement of Lactate Dehydrogenase activity in a range of biological samples.

    • Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)
      Functional Studies - Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (ab197000)

      Relative LDH Activity was calculated in lysates prepared from rat liver (0.037 µg protein), Jurkat cells (0.053 µg protein), and Human serum (0.2 µg protein).

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab197000? Please let us know so that we can cite the reference in this datasheet.

    ab197000 has been referenced in 6 publications.

    • Zhao Y  et al. Clinical features of cardiac lymphoma: an analysis of 37 cases. J Int Med Res 49:300060521999558 (2021). PubMed: 33752450
    • Madungwe NB  et al. Inner mitochondrial membrane protein MPV17 mutant mice display increased myocardial injury after ischemia/reperfusion. Am J Transl Res 12:3412-3428 (2020). Mouse . PubMed: 32774709
    • Dewhurst RM  et al. Development of Natural-Based Bone Cement for a Controlled Doxorubicin-Drug Release. Front Bioeng Biotechnol 8:754 (2020). PubMed: 32733869
    • SevIm Ç  et al. In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line. Turk J Pharm Sci 17:506-510 (2020). PubMed: 33177931
    • Xu W  et al. LyP-1-Modified Oncolytic Adenoviruses Targeting Transforming Growth Factor ß Inhibit Tumor Growth and Metastases and Augment Immune Checkpoint Inhibitor Therapy in Breast Cancer Mouse Models. Hum Gene Ther 31:863-880 (2020). PubMed: 32394753
    • Luengas-Martinez A  et al. Vascular Endothelial Growth Factor Blockade Induces Dermal Endothelial Cell Apoptosis in a Clinically Relevant Skin Organ Culture Model. Skin Pharmacol Physiol 33:110-118 (2020). PubMed: 32570235

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