Product nameAnti-Lactoferrin antibody
See all Lactoferrin primary antibodies
DescriptionGoat polyclonal to Lactoferrin
Tested applicationsSuitable for: IHC-P, WB, ELISA, ICCmore details
Species reactivityReacts with: Cow
Immunogen is purified lactoferrin of bovine milk origin
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntiserum was solid phase adsorbed to ensure specificity. ab112968 was isolated by affinity chromatography using antigen coupled to agarose beads.
Our Abpromise guarantee covers the use of ab112968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/2000.|
|WB||1/1000 - 1/30000. Predicted molecular weight: 78 kDa. Colorimetric: Use 1/1000-1/10000 dilution; Chemiluminescent: Use 1/1000-1/30000 dilution.|
|ELISA||1/100 - 1/30000. Coating: Use 1/100-1/500 dilution; Primary: Use 1/1000-1/30000 dilution.|
|ICC||1/200 - 1/2000.|
FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
Sequence similaritiesBelongs to the transferrin family.
Contains 2 transferrin-like domains.
- Information by UniProt
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All lanes : Anti-Lactoferrin antibody (ab112968) at 1/1000 dilution
Lane 1 : Resuspended cow lactoferrin powder at 2 µg
Lane 2 : Resuspended cow lactoferrin powder at 1 µg
Lane 3 : Resuspended cow lactoferrin powder at 0.5 µg
Lane 4 : Resuspended cow lactoferrin powder at 0.1 µg
Lane 5 : Resuspended cow lactoferrin powder at 0.05 µg
Lane 6 : Resuspended cow lactoferrin powder at 0.01 µg
All lanes : Rabbit Anti-Goat IgG H&L (HRP) (ab6741) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Blocked with 3% BSA for 1 hour at 20°C.
Incubated with the primary antibody for 16 hours at 4°C in blocking buffer.
Membrane - Nitrocellulose.