Application
Western blot
Sample
Cow Purified protein (Resuspended cow lactoferrin powder)
Gel Running Conditions
Reduced Denaturing (4% stacking 10% resolving)
Loading amount
2 µg
Specification
Resuspended cow lactoferrin powder
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Dilution
1/1000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 80 kDa
Positive control
Resuspended cow lactoferrin powder
Additional data
Additional Notes
Lane 1=2 ug Lane 2=1 ug Lane 3=0,5 ug Lane 4=0,1 ug Lane 5=0,05 ug Lane 6=0,01 ug
Molecular weight=80 kDa
Membrane Type: Nitrocellulose
Dr. Federico Benetti
Verified customer
Submitted May 02 2017