Overview

  • Product name

  • Description

    Rabbit polyclonal to Lactoferrin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant Human Lactoferrin protein

  • Positive control

    • MDA-MB231 cell line and mouse spleen tissue lysates; MDA-MB231 cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab135710 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab135710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 78 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • Sequence similarities

    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • GIG12 antibody
    • Growth inhibiting protein 12 antibody
    • HLF2 antibody
    • Kaliocin 1 antibody
    • Lactoferrin antibody
    • Lactoferroxin-C antibody
    • Lactotransferrin antibody
    • LF antibody
    • LTF antibody
    • Neutrophil lactoferrin antibody
    • Talalactoferrin antibody
    • TRFL_HUMAN antibody
    see all

Images

  • Anti-Lactoferrin antibody (ab135710) at 1/100 dilution + MDA-MB231 cell line lysate at 35 µg

    Predicted band size: 78 kDa

  • Anti-Lactoferrin antibody (ab135710) at 1/100 dilution + mouse spleen tissue lysate at 35 µg

    Predicted band size: 78 kDa

  • Flow Cytometric analysis of MDA-MB231 cells labelling Lactoferrin with ab135710 at 1/10 dilution (right histogram), compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

This product has been referenced in:

  • Sturge CR  et al. Cutting Edge: Developmental Regulation of IFN-? Production by Mouse Neutrophil Precursor Cells. J Immunol 195:36-40 (2015). Read more (PubMed: 26026057) »
  • Ménoret A  et al. Trace Levels of Staphylococcal Enterotoxin Bioactivity Are Concealed in a Mucosal Niche during Pulmonary Inflammation. PLoS One 10:e0141548 (2015). Read more (PubMed: 26509442) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your reply.

Unfortunately, as I was mentioning in my previous email, the antibodies we have tested in mouse were raised against the full length protein. Thus, we do not know the binding sites and such the potential cross-reactivity with transferrin. And those lactoferrin antibodies raised against a peptide (where we would know the binding site) do not work with mouse. Thus, we do not seem to have an antibody that matches your criteria. I am sorry to have no better news.

Please be sure to submit your data for ab38155 as Abreview so that you can receive the discount on your next order.

Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for your reply.

I was wondering how did you confirm that the lactoferrin antibody crossreacts with transferrin? The MW of both mouse proteins (serotransferrin in particular) are almost identical (78 vs 77 kDa). These cannot be distinguished by WB, unless a knockout or deletion experiment is being performed. Also, crossreactivity should be minimal as the homology is only 55%.

Since the antibodies are raised against the full length proteins, we do not know where the epitope regions are as they have not been mapped. If the epitope regions align well with a region from serotransferrin, then crossreactivity might be possible.

Also, for ab135710 the epitope regions of this rabbit poyclonal antibody have not been mapped. Thus, we cannot predict if cross reactivity might be likely or not.

You would need an antibody that was raised against a peptide so that cross reactivity can be predicted more precisely.We have 2 antibodies that were raised against petides, but they do not work in mouse or rat samples: ab109000 and ab109216. They are rabbit monoclonal antibodies.

How did you tests wit ab38155 turn out - you wanted to test this antibody in mouse samples? The datasheet says that it does not crossreact with transferrin.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

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