Overview

  • Product name
  • Description
    Rabbit polyclonal to Lactoferrin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ELISA, WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human).

  • Positive control
    • WB: Human lactoferrin.
  • General notes


    Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Antibody stabilization buffer.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity purified using immobilized antigenic proteins and pre adsorbed on an immobilized heterologus lactoferin matrix to remove the cross reacting antibodies.
  • Primary antibody notes
    Lactoferrin is an iron binding glycoprotein with an approximate molecular weight of 80 kDa. The protein has two iron binding domains each housing one Fe3+ and the synergistic CO32- ion. The crystal structure form of human lactoferrin at 2.2A resolution exhibits 5330 protein atoms, 2Fe2+, 2CO32- and 98 carbohydrate atoms. Lactoferrin is absorbed from intestine by apical side of the membrane and localized to the nuclei. Intravenous infusion of lactoferrin is protective against lethal doses of E coli and induce bacterimia by a mechanism that downregulates neutrophil TNF alfa secretion. Recombinant human lactoferrin (rhLF), expressed and extracted from rice seed, is being evaluated for use as a dietary supplement to treat iron deficiency and/or iron deficiency induced anemia. Lactoferrin has been shown to have a role in the immune system and in early development of the embryo. A specific receptor for lactoferrin binding has been implicated in the human fetal intestine. Early embryonic localisation of lactoferrin by IHC has suggested its presence in various tissues including intestinal epitheliuem, kiney, and various regions of the brain.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration. PubMed: 17244155
WB 1/500. Predicted molecular weight: 79 kDa.
IP 1/200.
ICC Use at an assay dependent concentration.

Target

  • Function
    Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
    Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
    Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
    The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
  • Sequence similarities
    Belongs to the transferrin family.
    Contains 2 transferrin-like domains.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • GIG12 antibody
    • Growth inhibiting protein 12 antibody
    • HLF2 antibody
    • Kaliocin 1 antibody
    • Lactoferrin antibody
    • Lactoferroxin-C antibody
    • Lactotransferrin antibody
    • LF antibody
    • LTF antibody
    • Neutrophil lactoferrin antibody
    • Talalactoferrin antibody
    • TRFL_HUMAN antibody
    see all

Images

  • Anti-Lactoferrin antibody (ab15811) at 1/500 dilution + Human Lactoferrin

    Predicted band size: 79 kDa
    Observed band size: 99 kDa (why is the actual band size different from the predicted?)

  • ab15811 at a 1/150 dilution staining Lactoferrin in human tonsil and intestinal tissue sections by Immunohistochemistry (paraffin embedded) incubated for 20 minutes at 25°C. Fixed with formaldehyde, enzymatic antigen retrieval step performed using proteinase K for 5 minutes at 37°C. Secondary used undiluted polyclonal Goat anti-mouse/rabbit IgG.

    See Abreview

References

This product has been referenced in:
  • Witzel M  et al. Chromatin-remodeling factor SMARCD2 regulates transcriptional networks controlling differentiation of neutrophil granulocytes. Nat Genet 49:742-752 (2017). IHC-P ; Human . Read more (PubMed: 28369036) »
  • Regan JK  et al. Damage-Associated Molecular Pattern and Fetal Membrane Vascular Injury and Collagen Disorganization in Lipopolysaccharide-Induced Intra-amniotic Inflammation in Fetal Sheep. Reprod Sci 23:69-80 (2016). IHC-P . Read more (PubMed: 26156854) »

See all 12 Publications for this product

Customer reviews and Q&As

Application
ELISA
Sample
Human Cell (stool)
Specification
stool
Type
Sandwich (Capture)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 22°C
Username

Mrs. Vanessa Voegele

Verified customer

Submitted Apr 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tonsil, intestine)
Specification
tonsil, intestine
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: proteinase K, 5 min, 37C
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Dec 29 2009

Dear Kol, Thank you for your enquiry. The composition of the Ab15811 (lactoferrin Ab) storage buffer is: PBS, glycerol and stabilizing proteins, pH 7.2-7.6. The buffer contains free amines and therefore can not be used for coupling through...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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