Key features and details
- Goat polyclonal to Lactoferrin
- Suitable for: ELISA, WB, Sandwich ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Lactoferrin antibody
See all Lactoferrin primary antibodies
DescriptionGoat polyclonal to Lactoferrin
Tested applicationsSuitable for: ELISA, WB, Sandwich ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
- Human Peripheral Blood Mononucleocyte lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab77548 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
Our Abpromise guarantee covers the use of ab77548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 85 kDa.
A 1 hour primary incubation is recommended for this product.
|Sandwich ELISA||Use a concentration of 5 - 10 µg/ml.|
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity.
Sequence similaritiesBelongs to the transferrin family.
Contains 2 transferrin-like domains.
- Information by UniProt
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Anti-Lactoferrin antibody (ab77548) at 0.05 µg/ml + Human Peripheral Blood Mononucleocyte lysate (in RIPA buffer) at 35 µg
Observed band size: 85 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab77548 (2µg/ml) staining of paraffin embedded Human Blood Vessel shows strong staining of cytoplasm of neutrophils. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Sandwich-type ELISA with increasing amount of recombinant LTF captured by a rabbit antibody. ab77548 reporter concentration: 5 ug/ml
ab77548 has been referenced in 1 publication.
- Alhalwani AY et al. Development of a sandwich ELISA with potential for selective quantification of human lactoferrin protein nitrated through disease or environmental exposure. Anal Bioanal Chem 410:1389-1396 (2018). PubMed: 29214534