Product nameLactose Assay Kit
Sample typeMilk, Other biological fluids
Assay time1h 00m
In Abcam's Lactose Assay Kit Lactose is hydrolyzed to glucose and galactose. The galactose is subsequently oxidized generating color (OD570nm) and fluorescence (Ex/Em 535/587 nm). Free galactose can be corrected by a background control in the absence of lactase. The Lactose Assay Kit provides a simple, convenient, and sensitive means for direct measurement of lactose levels in various biological samples (serum, plasma, other body fluids, food, growth media, etc.). Pretreatment of samples is not required. The kit can be used as a high throughput assay.
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Lactose (C12H22O11 FW: 342.3) is an important naturally occurred disaccharide, consisting of one galactose and one glucose. Milk contains ~2-8% lactose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests HRP (Lyophilized) Purple 1 vial Lactase (Lyophilized) Blue 1 vial Lactose Assay Buffer WM 1 x 25ml Lactose Enzyme Mix (Lyophilized) Green 1 vial Lactose Standard (100 nmol/ul) Yellow 1 x 100µl Probe (DMSO solution) Red 1 x 200µl
RelevanceLactose (C12H22O11 FW: 342.3) is an important naturally occurring disaccharide along with sucrose and maltose. Lactose makes up around 2-8% of milk. It consists of one galactose and one glucose. Some people, particularly infants, lack the enzyme necessary to digest galactose leading to galactose accumulation in blood (Galactosemia) causing enlarged liver, renal failure, cataracts and brain damage.
- Milk sugar
ab83384 has been referenced in 5 publications.
- Lee S et al. A genetic variant in SLC30A2 causes breast dysfunction during lactation by inducing ER stress, oxidative stress and epithelial barrier defects. Sci Rep 8:3542 (2018). PubMed: 29476070
- Rudolph MC et al. Thyroid hormone responsive protein Spot14 enhances catalysis of fatty acid synthase in lactating mammary epithelium. J Lipid Res 55:1052-65 (2014). PubMed: 24771867
- Gregor MF et al. The role of adipocyte XBP1 in metabolic regulation during lactation. Cell Rep 3:1430-9 (2013). PubMed: 23623498
- Wahlig JL et al. Impact of high-fat diet and obesity on energy balance and fuel utilization during the metabolic challenge of lactation. Obesity (Silver Spring) 20:65-75 (2012). PubMed: 21720435
- Dempsey C et al. Marginal maternal zinc deficiency in lactating mice reduces secretory capacity and alters milk composition. J Nutr 142:655-60 (2012). Mouse . PubMed: 22357740