Overview

  • Product name

    Lactulose Assay Kit (Fluorometric)
  • Detection method

    Fluorescent
  • Sample type

    Milk
  • Assay type

    Quantitative
  • Range

    0.01 nmol/well - 1 nmol/well
  • Product overview

    Lactulose Assay Kit (Fluorometric) (ab196986) is a simple, sensitive and convenient assay to quantify lactulose in non-biological samples such as pharmaceuticals, foods, and dairy products. Lactulose present in the sample is hydroyzed by the Lactulose Enzyme Mix to form galactose and fructose. Production of fructose can be measured in a fluorescence plate reader (Ex/Em = 535/587 nm) and the amount of fructose is directly proportional to the amount of lactulose present in the sample. On the other hand, traditional lactulose assays are based on detecting the amount of glucose formed from fructose, leading to incresaed background level in the assay.

    The linear range of the assay is between approximately 10 pmol and 1 nmol of lactulose.

  • Notes

    Lactulose is a synthetic disaccharide composed of galactose and fructose. It is closely related to lactose which is a disaccharide consisting of galactose and glucose. Lactulose is not absorbed by the gut and it is therefore commonly used as a laxative and for hepatic encephalopathy. It can also be found in heated milk since it is easily formed by isomerization of lactose at elevated temperatures or in alkaline solutions.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Enhancement Solution 1 x 1.5ml
    Hydrolysis Enzyme Mix (lyophilized) Blue 1 vial
    Lactulose Enzyme Mix 1 vial
    Lactulose Hydrolysis Buffer 1 x 25ml
    Lactulose Reaction Buffer 1 x 25ml
    Lactulose Standard (100 mM) 1 x 80µl
    PicoProbe Blue 1 x 300µl

Associated products

Images

  • Left: Standard Curve of Lactulose before and after enhancement (not corrected for background). Right: Results obtained for milk: 100 µL were treated with Carrez Clarification Reagent, then 10 µL were diluted 1:20 with Lactulose Hydrolysis Buffer and analyzed following this protocol.

  • Time course of Standard Curve of 0-1 nmol lactulose before enhancement and signal after enhancement.

Protocols

References

ab196986 has not yet been referenced specifically in any publications.

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