• Product name

    Anti-LAG-3 antibody [11E3]
    See all LAG-3 primary antibodies
  • Description

    Mouse monoclonal [11E3] to LAG-3
  • Host species

  • Specificity

    Recognizes the first N-terminal D1 domain of LAG-3.

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, IP, WB, ELISA, ICC/IF, IHC-Fr, Blockingmore details
  • Species reactivity

    Reacts with: Human, Monkey
  • Immunogen

    Recombinant full length protein corresponding to Human LAG-3. (soluble form)

  • General notes

    This product was previously labelled as Lymphocyte Activation Gene 3.



Our Abpromise guarantee covers the use of ab40465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.
IHC-P Use at an assay dependent concentration.
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 58 kDa.
ELISA Use a concentration of 5 µg/ml.
ICC/IF 1/100.
IHC-Fr 1/150.
Blocking Use at an assay dependent concentration.


  • Function

    Involved in lymphocyte activation. Binds to HLA class-II antigens.
  • Tissue specificity

    On cell surface of activated NK and T-lymphocytes.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • CD223 antibody
    • CD223 antigen antibody
    • FDC protein antibody
    • LAG-3 antibody
    • Lag3 antibody
    • LAG3_HUMAN antibody
    • Lymphocyte activating 3 antibody
    • Lymphocyte activation gene 3 antibody
    • Lymphocyte activation gene 3 protein antibody
    • Protein FDC antibody
    see all


  • ab40465 staining LAG-3 in Human submandibular gland (disease) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with blocking buffer for 5 minutes at 24°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/100 in PBS) for 2 hours at 25°C. An undiluted Biotin-conjugated anti-mouse IgG monoclonal was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing Jurkat cells stained with ab40465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40465, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


This product has been referenced in:

  • Lee WJ  et al. Expression of lymphocyte-activating gene 3 and T-cell immunoreceptor with immunoglobulin and ITIM domains in cutaneous melanoma and their correlation with programmed cell death 1 expression in tumor-infiltrating lymphocytes. J Am Acad Dermatol N/A:N/A (2019). Read more (PubMed: 30880064) »
  • Takada K  et al. Use of the tumor-infiltrating CD8 to FOXP3 lymphocyte ratio in predicting treatment responses to combination therapy with pertuzumab, trastuzumab, and docetaxel for advanced HER2-positive breast cancer. J Transl Med 16:86 (2018). IHC-P ; Human . Read more (PubMed: 29615076) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (FFPE HDLM2 cells pellet)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6
FFPE HDLM2 cells pellet
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 22°C

Zohar Gavish

Verified customer

Submitted Dec 12 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
DAKO Blocking buffer as blocking agent for 5 minute(s) · Concentration: 0% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer (pH6.0)
Human Tissue sections (submandibular gland (disease))
submandibular gland (disease)

Abcam user community

Verified customer

Submitted Dec 23 2013


Thank you very much calling Abcam earlier today. Below is the information about our testing discount program that I talked about.

Asab40465 has not been tested in Flow Cytometry. Therefore, I can offer a discount off a future purchase if you buy ab40465 now, test it in Flow Cytometry and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free (PRIMARY ANTIBODY/PROTEIN) OR VALUE OFF OF NEXT ORDER.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab40465 in Flow Cytometry. I will then send a discount code. This code must be issued before purchasing ab40465 so please wait for my reply before ordering.

2. Purchase ab40465 either by phone, fax, or online (www.abcam.com).

3. Test it in Flow Cytometry.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab40465 turns out to be unsuitable for Flow Cytometry, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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For licensing inquiries, please contact partnerships@abcam.com

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