Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR027F] to LAG-3
- Suitable for: WB, ICC, IHC-P, IP
- Reacts with: Human
Product nameAnti-LAG-3 antibody [BLR027F]
See all LAG-3 primary antibodies
DescriptionRabbit monoclonal [BLR027F] to LAG-3
Tested applicationsSuitable for: WB, ICC, IHC-P, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human LAG-3 aa 500-525 (C terminal). The exact sequence is proprietary. NP_002277.4 and Gene ID 3902.
Database link: P18627
This product is sold under License from Bethyl Laboratories, Inc.
This product was previously labelled as Lymphocyte Activation Gene 3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/100 - 1/500.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.
Use 12µl/0.5mg lysate.
FunctionInvolved in lymphocyte activation. Binds to HLA class-II antigens.
Tissue specificityOn cell surface of activated NK and T-lymphocytes.
Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- CD223 antibody
- CD223 antigen antibody
- FDC protein antibody
LAG-3 was immunoprecipitated from 0.5 mg HDLM-2 whole cell lysate with ab243884 at 12 µl per reaction. Western blot was performed on the immunoprecipitate using ab243884 at 1/1000 dilution.
Lane 1: ab243884 IP in HDLM-2 whole cell lysate.
Lane 2: Control IgG IP in HDLM-2 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Western blot analysis using ab243884 at 1/1000 dilution.
Lane 1: HDLM-2 whole cell lysate (50 µg).
Lane 2: RPMI 8226 whole cell lysate (50 µg).
Lane3: Jurkat whole cell lysate (50 µg).
Lane 4: MJ whole cell lysate (50 µg).
Lane 5: HEK-293T whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
Formalin-fixed, paraffin-embedded HDLM-2 cells labeling LAG-3 using ab243884 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 using ab243884 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243884 has not yet been referenced specifically in any publications.