Recombinant

Recombinant Anti-LAG-3 antibody [BLR027F] (ab243884)

Overview

  • Product name

    Anti-LAG-3 antibody [BLR027F]
    See all LAG-3 primary antibodies
  • Description

    Rabbit monoclonal [BLR027F] to LAG-3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human LAG-3 aa 500-525 (C terminal). The exact sequence is proprietary. NP_002277.4 and Gene ID 3902.
    Database link: P18627

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc.

    This product was previously labelled as Lymphocyte Activation Gene 3.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab243884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000.
ICC 1/100 - 1/500.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration.

Use 12µl/0.5mg lysate.

Target

  • Function

    Involved in lymphocyte activation. Binds to HLA class-II antigens.
  • Tissue specificity

    On cell surface of activated NK and T-lymphocytes.
  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD223 antibody
    • CD223 antigen antibody
    • FDC protein antibody
    • LAG-3 antibody
    • Lag3 antibody
    • LAG3_HUMAN antibody
    • Lymphocyte activating 3 antibody
    • Lymphocyte activation gene 3 antibody
    • Lymphocyte activation gene 3 protein antibody
    • Protein FDC antibody
    see all

Images

  • LAG-3 was immunoprecipitated from 0.5 mg HDLM-2 whole cell lysate with ab243884 at 12 µl per reaction. Western blot was performed on the immunoprecipitate using ab243884 at 1/1000 dilution.

    Lane 1: ab243884 IP in HDLM-2 whole cell lysate.

    Lane 2: Control IgG IP in HDLM-2 whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

  • Western blot analysis using ab243884 at 1/1000 dilution.

    Lane 1: HDLM-2 whole cell lysate (50 µg).

    Lane 2: RPMI 8226 whole cell lysate (50 µg).

    Lane3: Jurkat whole cell lysate (50 µg).

    Lane 4: MJ whole cell lysate (50 µg).

    Lane 5: HEK-293T whole cell lysate (50 µg).

    A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.

  • Formalin-fixed, paraffin-embedded HDLM-2 cells labeling LAG-3 using ab243884 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 using ab243884 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

References

ab243884 has not yet been referenced specifically in any publications.

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