Product nameAnti-LAG-3 antibody [BLR027F]
See all LAG-3 primary antibodies
DescriptionRabbit monoclonal [BLR027F] to LAG-3
Tested applicationsSuitable for: WB, ICC, IHC-P, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human LAG-3 aa 500-525 (C terminal). The exact sequence is proprietary. NP_002277.4 and Gene ID 3902.
Database link: P18627
This product is sold under License from Bethyl Laboratories, Inc.
This product was previously labelled as Lymphocyte Activation Gene 3.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 8.2
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notesRecombinant antibody was purified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab243884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||1/100 - 1/500.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.
Use 12µl/0.5mg lysate.
FunctionInvolved in lymphocyte activation. Binds to HLA class-II antigens.
Tissue specificityOn cell surface of activated NK and T-lymphocytes.
Sequence similaritiesContains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- CD223 antibody
- CD223 antigen antibody
- FDC protein antibody
LAG-3 was immunoprecipitated from 0.5 mg HDLM-2 whole cell lysate with ab243884 at 12 µl per reaction. Western blot was performed on the immunoprecipitate using ab243884 at 1/1000 dilution.
Lane 1: ab243884 IP in HDLM-2 whole cell lysate.
Lane 2: Control IgG IP in HDLM-2 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Western blot analysis using ab243884 at 1/1000 dilution.
Lane 1: HDLM-2 whole cell lysate (50 µg).
Lane 2: RPMI 8226 whole cell lysate (50 µg).
Lane3: Jurkat whole cell lysate (50 µg).
Lane 4: MJ whole cell lysate (50 µg).
Lane 5: HEK-293T whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
Formalin-fixed, paraffin-embedded HDLM-2 cells labeling LAG-3 using ab243884 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 using ab243884 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
ab243884 has not yet been referenced specifically in any publications.