Recombinant Anti-LAG-3 antibody [CAL77] (ab237720)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CAL77] to LAG-3
- Suitable for: Flow Cyt (Intra), IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-LAG-3 antibody [CAL77]
See all LAG-3 primary antibodies -
Description
Rabbit monoclonal [CAL77] to LAG-3 -
Host species
Rabbit -
Specificity
IHC-P is only recommended for human samples. We obtained negative staining results with mouse.
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Tested applications
Suitable for: Flow Cyt (Intra), IHC-Pmore details
Unsuitable for: ICC/IF,IP or WB -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil tissue. Flow Cyt (intra): Human PBMC. Mouse splenocytes treated with ConA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purity is greater than 99%. -
Clonality
Monoclonal -
Clone number
CAL77 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab237720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/500.
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IHC-P |
1/900. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/500. |
IHC-P
1/900. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Involved in lymphocyte activation. Binds to HLA class-II antigens. -
Tissue specificity
On cell surface of activated NK and T-lymphocytes. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3902 Human
- Entrez Gene: 16768 Mouse
- Omim: 153337 Human
- SwissProt: P18627 Human
- SwissProt: Q61790 Mouse
- Unigene: 409523 Human
- Unigene: 4528 Mouse
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Alternative names
- CD223 antibody
- CD223 antigen antibody
- FDC protein antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LAG-3 with ab237720 at 1/900 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the human tonsil is observed. The section was incubated with ab237720 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 30 mins.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 with ab237720 at 0.25 µg/ml in immunohistochemical analysis.
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Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized human peripheral blood mononuclear cell (PBMC) treated with 10μg/ml PHA for 2 days, labeling LAG-3 with ab237720 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD3 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellular staining with rabbit IgG (Left) and ab237720 (Right).
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Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1%Tween-20 permeabilized mouse splenocytes treated with 2.5µg/ml Concanavalin A (ConA) for 3 days, labeling LAG-3 with ab237720 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD3 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellular staining with rabbit IgG (Left) and ab237720 (Right).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab237720 has not yet been referenced specifically in any publications.